Validation Data Gallery
|Positive WB detected in
|HCT 116 cells, mouse testis tissue, human kidney tissue, human placenta tissue, human brain tissue, rat testis tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, MDA-MB-231 cells, C2C12 cells, NIH/3T3 cells
|Positive IP detected in
|Positive IHC detected in
|human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:2000-1:16000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
11380-1-AP targets DDB1 in WB, IP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|DDB1 fusion protein Ag1901
|damage-specific DNA binding protein 1, 127kDa
|Calculated molecular weight
|1140 aa, 127 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1.
Ddb1 Is Essential for the Expansion of CD4+ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.
A Multidimensional Characterization of E3 Ubiquitin Ligase and Substrate Interaction Network.
14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage.
DDB1 is a cellular substrate of NS3/4A protease and required for hepatitis C virus replication.
Cell Death Dis
Cul4 E3 ubiquitin ligase regulates ovarian cancer drug resistance by targeting the antiapoptotic protein BIRC3.
SENP3 and USP7 regulate Polycomb-rixosome interactions and silencing functions
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sarah (Verified Customer) (07-03-2019)
Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-DDB1 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.