DOPA decarboxylase/DDC Polyclonal antibody

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  WB analysis using 10166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10166-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10166-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10166-1-AP

  IP result of anti-DOPA decarboxylase/DDC (IP:10166-1-AP, 4ug; Detection:10166-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase/DDC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10166-1-AP (DOPA decarboxylase/DDC antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10166-1-AP (DOPA decarboxylase/DDC antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of SH-SY5Y using 10166-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DOPA decarboxylase antibody (10166-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DOPA decarboxylase/DDC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10166-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

DDC, DOPA decarboxylase, AADC, Aromatic-L-amino-acid decarboxylase, EC:4.1.1.28

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse kidney tissue, mouse brain tissue, rat kidney tissue, PC-12 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse kidney tissue, human liver cancer tissue, rat kidney tissue, rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Product Information

10166-1-AP targets DOPA decarboxylase/DDC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOPA decarboxylase/DDC fusion protein Ag0219
• Full Name: dopa decarboxylase (aromatic L-amino acid decarboxylase)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC008366
• Gene Symbol: DOPA decarboxylase
• Gene ID (NCBI): 1644
• RRID: AB_2088937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20711
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOPA decarboxylase (DDC), also known as aromatic l-amino acid decarboxylase, belongs to the pyridoxal-dependent aminotransferase superfamily. DDC is an enzyme that converts levodopa into dopamine14, the latter being severely depleted in LBD due to the loss of dopaminergic neurons in the substantia nigra (PMID: 3374198, PMID: 28100251). DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.

Publications

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse,human	WB,IHC	Cell Signal; Profile of 5-HT2A receptor involved in signaling cascades associated to intracellular inflammation and apoptosis in hepatocytes and its role in carbon tetrachloride-induced hepatotoxicity; Authors - Yuxin Zhang
human	WB	Dis Model Mech; A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease.; Authors - Zhao-Feng Li
mouse	IHC,IF	Toxicol Appl Pharmacol; PMID: 21703292; Authors - Fox Donald A DA
human	WB	J Transl Med; Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease.; Authors - Ming Ming
mouse,human	WB,IHC	Toxicol Appl Pharmacol; Carbon tetrachloride induced mitochondrial division, respiratory chain damage, abnormal intracellular [H+] and apoptosis are due to the activation of 5-HT degradation system in hepatocytes.; Authors - Yuxin Zhang