DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Recombinant Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (1)

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

CloneNo.

4K14

Cat no : 80010-1-RR

Synonyms

DDDDK, DDDDK tag, DYKDDDDK, Flag, Flag tag



Tested Applications

Positive WB detected inTDP43-PCDNA6, PCSK9
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inHEK-293 cells, Transfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

80010-1-RR targets DYKDDDDK tag in WB, IP, IF,ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DYKDDDDK tag fusion protein Ag2329
Full Name DYKDDDDK tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protocols

Product Specific Protocols
WB protocol for DYKDDDDK tag antibody 80010-1-RRDownload protocol
IF protocol for DYKDDDDK tag antibody 80010-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Death Dis

ADCK1 activates the β-catenin/TCF signaling pathway to promote the growth and migration of colon cancer cells.

Authors - Yong Ji
WB

Cell Commun Signal

FAK displacement from focal adhesions: a promising strategy to target processes implicated in cancer progression and metastasis.

Authors - Ioanna Antoniades
WB

Front Cell Dev Biol

Urea as a By-Product of Ammonia Metabolism Can Be a Potential Serum Biomarker of Hepatocellular Carcinoma.

Authors - Changsen Bai
humanWB

Pharmacol Res

Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect.

Authors - Dongrong Zhu