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CHOP; GADD153 Polyclonal antibody

CHOP; GADD153 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IF, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 15204-1-AP

Synonyms

C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP; GADD153, CHOP10, DDIT 3, DDIT3, GADD153



Tested Applications

Positive WB detected inTunicamycin treated HeLa cells, HeLa cells, MCF-7 cells, K-562 cells, C6 cells, RAW 264.7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inC6 cells
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue, human breast cancer tissue, human thyroid cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inTunicamycin treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15204-1-AP targets CHOP; GADD153 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, zebrafish, Hamster, pig, Bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHOP; GADD153 fusion protein Ag7354
Full Name DNA-damage-inducible transcript 3
Calculated Molecular Weight 19 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC003637
Gene Symbol CHOP
Gene ID (NCBI) 1649
RRIDAB_2292610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Protocols

Product Specific Protocols
WB protocol for CHOP; GADD153 antibody 15204-1-APDownload protocol
IHC protocol for CHOP; GADD153 antibody 15204-1-APDownload protocol
IF protocol for CHOP; GADD153 antibody 15204-1-APDownload protocol
IP protocol for CHOP; GADD153 antibody 15204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J
humanWB

Mol Cell

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis

Authors - Yi Fu
mouseIF

Acta Neuropathol

Wolframin is a novel regulator of tau pathology and neurodegeneration.

Authors - Shuo Chen
humanWB,IHC,IP,ChIP

Mol Cell

The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma.

Authors - Hayley J Zullow
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
mouse,ratWB

Cell Death Differ

Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation

Authors - Guangjin Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Fan (Verified Customer) (03-22-2021)

Mouse retinal tissue was used. Single band detected at the right size.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
CHOP; GADD153 Antibody Western Blot validation (1:500 dilution) in (Cat no:15204-1-AP)
FH

Daniel (Verified Customer) (06-25-2019)

Specific staining detected in the proximal tubules.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse kidney (TRACK model)
CHOP; GADD153 Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse kidney (TRACK model) (Cat no:15204-1-AP)