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  • KD/KO Validated

CHOP; GADD153 Polyclonal antibody

CHOP; GADD153 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF, FC, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 15204-1-AP

Synonyms

CEBPZ, CHOP, CHOP 10, DDIT 3, DDIT3, GADD153



Tested Applications

Positive WB detected inTunicamycin treated HeLa cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

15204-1-AP targets CHOP; GADD153 in WB, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Bovine, chicken, hamster, human, mouse, pig, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHOP; GADD153 fusion protein Ag7354
Full Name DNA-damage-inducible transcript 3
Calculated molecular weight 19 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC003637
Gene symbol DDIT3
Gene ID (NCBI) 1649
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Protocols

Product Specific Protocols
WB protocol for CHOP; GADD153 antibody 15204-1-APDownload protocol
IHC protocol for CHOP; GADD153 antibody 15204-1-APDownload protocol
FC protocol for CHOP; GADD153 antibody 15204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Nanomedicine

A comparative study of toxicity of TiO2, ZnO, and Ag nanoparticles to human aortic smooth-muscle cells.

Authors - Maolin Wang
human,mouseWB

Br J Pharmacol

Betulinic acid alleviates endoplasmic reticulum stress-mediated NAFLD through activation of FXR in mice.

Authors - Ming Gu
ratIHC

Acta Biochim Biophys Sin (Shanghai)

Chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and nonalcoholic steatohepatitis via angiogenesis rather than endoplasmic reticulum stress.

Authors - Wei Wu
ratWB

Br J Pharmacol

Hydrogen Sulfide Regulates Muscle RING Finger-1 Protein S-Sulfhydration at Cys44 to Prevent Cardiac Structural Damage in Diabetic Cardiomyopathy.

Authors - Xiaojiao Sun
mouseIHC

J Cell Sci

Tmem30a Deficiency in endothelial cells impairs cell proliferation and angiogenesis.

Authors - Shanshan Zhang
pigWB

Int J Biol Macromol

A pectic polysaccharide from water decoction of Xinjiang Lycium barbarum fruit protects against intestinal endoplasmic reticulum stress.

Authors - Chao Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Fan (Verified Customer) (03-22-2021)

Mouse retinal tissue was used. Single band detected at the right size.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
CHOP; GADD153 Antibody Western Blot validation (1:500 dilution) in (Cat no:15204-1-AP)
FH

Daniel (Verified Customer) (06-25-2019)

Specific staining detected in the proximal tubules.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse kidney (TRACK model)
CHOP; GADD153 Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse kidney (TRACK model) (Cat no:15204-1-AP)