DDIT4L Polyclonal antibody, PBS Only

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  WB analysis using 28490-1-AP (same clone as 28490-1-PBS)

  Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 28490-1-AP (DDIT4L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28490-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 28490-1-AP (same clone as 28490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 28490-1-AP (DDIT4L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28490-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 28490-1-AP (same clone as 28490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 28490-1-AP (DDIT4L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28490-1-PBS in a different storage buffer formulation.

DDIT4L Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28490-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

RTP801L, REDD-2, REDD2, HIF-1 responsive protein RTP801-like, DNA damage-inducible transcript 4-like protein

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Product Information

28490-1-PBS targets DDIT4L in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDIT4L fusion protein Ag29616
• Full Name: DNA-damage-inducible transcript 4-like
• Calculated Molecular Weight: 193 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC013592
• Gene Symbol: DDIT4L
• Gene ID (NCBI): 115265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96D03
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DNA-damage-inducible transcript 4-like protein(DDIT4L), encoded by the stress responsive gene REDD2, is a negative regulator of mTOR signaling, and expressed predominantly in skeletal muscle. It regulates the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Also, DDIT4L involves in oxidized low-density lipoprotein-induced macrophage death sensitivity.