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DDX46 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 16927-1-AP

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Con: 25 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

DDX46 Antibody WB HEK-293 cells 16927-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 16927-1-AP(DDX46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

DDX46 Antibody IF HepG2 cells 16927-1-AP

Immunofluorescent analysis of HepG2 cells, using DDX46 antibody 16927-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

DDX46 Antibody IP NIH/3T3 cells 16927-1-AP

IP Result of anti-DDX46 (IP:16927-1-AP, 4ug; Detection:16927-1-AP 1:1000) with NIH/3T3 cells lysate 2000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IP detected in:
NIH/3T3 cells
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
DDX46 fusion protein Ag10342
Full name:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
Calculated molecular weight:
1031aa,117 kDa
Observed molecular weight:
120-125 kDa
GenBank accession number:
BC012304
Gene ID (NCBI):
9879
Gene symbol
DDX46
Synonyms
DDX46, DEAD box protein 46, KIAA0801, PRP5 homolog, PRPF5

Background

DDX46, also named as KIAA0801, is a 1031 amino acid protein, which belongs to the DEAD box helicase family. DDX46 has been shown to be required at the early step of pre-spliceosome assembly. It uses the energy released by the hydrolysis of ATP to rearrange local RNA-RNA or protein-RNA interactions. The nuclear DDX46 acted as a negative regulator of the antiviral innate response by recruiting the m6A‘eraser’ ALKBH5 to induce retention of antiviral innate mRNA in the nucleus.


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