DDX46 Polyclonal antibody

DDX46 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16927-1-AP

Synonyms

DDX46, DEAD box protein 46, KIAA0801, PRP5 homolog, PRPF5

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Validation Data Gallery

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  WB analysis of HEK-293 using 16927-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16927-1-AP (DDX46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16927-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16927-1-AP (DDX46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16927-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16927-1-AP (DDX46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 16927-1-AP

  IP result of anti-DDX46 (IP:16927-1-AP, 4ug; Detection:16927-1-AP 1:1000) with NIH/3T3 cells lysate 2000ug.

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  IF Staining of HepG2 using 16927-1-AP

  Immunofluorescent analysis of HepG2 cells, using DDX46 antibody 16927-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

16927-1-AP targets DDX46 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX46 fusion protein Ag10342
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
• Calculated Molecular Weight: 1031 aa, 117 kDa
• Observed Molecular Weight: 120-125 kDa
• GenBank Accession Number: BC012304
• Gene Symbol: DDX46
• Gene ID (NCBI): 9879
• RRID: AB_2090927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX46, also named as KIAA0801, is a 1031 amino acid protein, which belongs to the DEAD box helicase family. DDX46 has been shown to be required at the early step of pre-spliceosome assembly. It uses the energy released by the hydrolysis of ATP to rearrange local RNA-RNA or protein-RNA interactions. The nuclear DDX46 acted as a negative regulator of the antiviral innate response by recruiting the m6A'eraser' ALKBH5 to induce retention of antiviral innate mRNA in the nucleus.

Publications

Species	Application	Title
human	IF	Elife; Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP.; Authors - Andrea Pawellek
human	WB	PLoS Pathog; Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival.; Authors - Liang Wei Wang
human	IF	Sci Rep; Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics.; Authors - Sunyuan Zhang
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu