Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, NIH/3T3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human stomach tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 2 publications below |
Product Information
16927-1-AP targets DDX46 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DDX46 fusion protein Ag10342 Predict reactive species |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 |
| Calculated Molecular Weight | 1031 aa, 117 kDa |
| Observed Molecular Weight | 120-125 kDa |
| GenBank Accession Number | BC012304 |
| Gene Symbol | DDX46 |
| Gene ID (NCBI) | 9879 |
| RRID | AB_2090927 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L014 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDX46, also named as KIAA0801, is a 1031 amino acid protein, which belongs to the DEAD box helicase family. DDX46 has been shown to be required at the early step of pre-spliceosome assembly. It uses the energy released by the hydrolysis of ATP to rearrange local RNA-RNA or protein-RNA interactions. The nuclear DDX46 acted as a negative regulator of the antiviral innate response by recruiting the m6A'eraser' ALKBH5 to induce retention of antiviral innate mRNA in the nucleus.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX46 antibody 16927-1-AP | Download protocol |
| IHC protocol for DDX46 antibody 16927-1-AP | Download protocol |
| IP protocol for DDX46 antibody 16927-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP. | ||
PLoS Pathog Epstein-Barr virus subverts mevalonate and fatty acid pathways to promote infected B-cell proliferation and survival. | ||
Sci Rep Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics. | ||
Cell Rep Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments. | ||
Neoplasma Oncogenic DDX46 promotes pancreatic cancer development and gemcitabine resistance by facilitating the JMJD6/CDK4 signaling pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Xiao (Verified Customer) (05-27-2025) | This antibody works very good for WB (HT1080, 293T cell lines), but not good for IP (HT1080, 293T cell lines) in my hand.
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