Desmin Polyclonal antibody
Desmin Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC
Conjugate
Unconjugated
Cat no : 22205-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
Positive IHC detected in | human heart tissue, human skeletal muscle tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 22205-1-AP is Desmin Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Desmin fusion protein Ag17525 |
Full Name | desmin |
Calculated molecular weight | 470 aa, 54 kDa |
Observed molecular weight | 50-55 kDa |
GenBank accession number | BC032116 |
Gene symbol | DES |
Gene ID (NCBI) | 1674 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.
Protocols
Product Specific Protocols | |
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WB protocol for Desmin antibody 22205-1-AP | Download protocol |
IHC protocol for Desmin antibody 22205-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |