Desmin Polyclonal antibody

Desmin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 22205-1-AP

Synonyms

CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin

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Validation Data Gallery

• 

  WB analysis of mouse heart using 22205-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22205-1-AP (Desmin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22205-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22205-1-AP (Desmin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 22205-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22205-1-AP (Desmin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 22205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22205-1-AP is Desmin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Desmin fusion protein Ag17525
• Full Name: desmin
• Calculated molecular weight: 470 aa, 54 kDa
• Observed molecular weight: 50-55 kDa
• GenBank accession number: BC032116
• Gene symbol: DES
• Gene ID (NCBI): 1674
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.