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  • KD/KO Validated

DGCR8 Monoclonal antibody

DGCR8 Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IP, RIP, ELISA

Conjugate

Unconjugated

CloneNo.

4D11A3

Cat no : 60084-1-Ig

Synonyms

C22orf12, DGCR8, DGCRK6, Gy1



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, BxPC-3 cells, RAW 264.7 cells, A431 cells, Jurkat cells, K-562 cells, ROS1728 cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60084-1-Ig targets DGCR8 in WB, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DGCR8 fusion protein Ag4871
Full Name DiGeorge syndrome critical region gene 8
Calculated Molecular Weight 773 aa, 86 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC009323
Gene Symbol DGCR8
Gene ID (NCBI) 54487
RRIDAB_2090985
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

Protocols

Product Specific Protocols
WB protocol for DGCR8 antibody 60084-1-IgDownload protocol
IP protocol for DGCR8 antibody 60084-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Nature

Global miRNA dosage control of embryonic germ layer specification.

Authors - Yingzi Cui
  • KO Validated
mouseWB

Mol Cell

A transient transcriptional activation governs unpolarized-to-polarized morphogenesis during embryo implantation

Authors - Xuehui Lyu
humanWB

Nat Commun

Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells.

Authors - Hailong Zhang
human,ratWB

Aging Cell

p38 MAPK-mediated loss of nuclear RNase III enzyme Drosha underlies amyloid beta-induced neuronal stress in Alzheimer's disease

Authors - Haidong Xu
humanWB,RIP

Mol Ther

DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.

Authors - Fangfang Zhang
human, mouseWB

Nucleic Acids Res

SUMOylation at K707 of DGCR8 controls direct function of primary microRNA.

Authors - Changhong Zhu