DGCR8 Monoclonal antibody

DGCR8 Monoclonal Antibody for IP, WB,ELISA

Host / Isotype

Mouse / IgG2a


human, mouse, rat, pig







Cat no : 60084-1-Ig


C22orf12, DGCR8, DGCRK6, Gy1

Tested Applications

Positive WB detected inK562 cells, HepG2 cells, BxPC-3 cells, human brain tissue, HEK-293 cells, RAW 264.7 cells, A431 cells
Positive IP detected inK-562 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

60084-1-Ig targets DGCR8 in WB, RIP, IP,ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DGCR8 fusion protein Ag4871
Full Name DiGeorge syndrome critical region gene 8
Calculated molecular weight 773 aa, 86 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC009323
Gene symbol DGCR8
Gene ID (NCBI) 54487
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.


Product Specific Protocols
WB protocol for DGCR8 antibody 60084-1-IgDownload protocol
IP protocol for DGCR8 antibody 60084-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Neurochem

Primary microRNA precursor transcripts are localized at post-synaptic densities in adult mouse forebrain.

Authors - Lugli Giovanni G


Identification of microprocessor-dependent cancer cells allows screening for growth-sustaining micro-RNAs.

Authors - Peric D D
human, mouseWB

Nucleic Acids Res

SUMOylation at K707 of DGCR8 controls direct function of primary microRNA.

Authors - Changhong Zhu

Mol Ther

DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.

Authors - Fangfang Zhang
  • KD Validated


Global miRNA dosage control of embryonic germ layer specification.

Authors - Yingzi Cui
  • KO Validated