DHFRL1 Polyclonal antibody

DHFRL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27965-1-AP

Synonyms

DHFR2, DHFRL1, DHFRP4, Dihydrofolate reductase 2, mitochondrial, dihydrofolate reductase like 1, FLJ16119

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Validation Data Gallery

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  WB analysis using 27965-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27965-1-AP (DHFRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 27965-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27965-1-AP (DHFRL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 27965-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27965-1-AP (DHFRL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27965-1-AP targets DHFRL1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHFRL1 fusion protein Ag10537
• Full Name: dihydrofolate reductase-like 1
• Calculated Molecular Weight: 187 aa, 22 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC063379
• Gene Symbol: DHFRL1
• Gene ID (NCBI): 200895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHFRL1, also known as DHFR2, is a key enzyme in folate metabolism. DHFRL1 is a dihydrofolate reductase, that catalyzes the NADPH-dependent reduction of dihydrofolate to the biologically active form, tetrahydrofolate (PMID: 21876184).