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  • KD/KO Validated

DHX58/LGP2 Polyclonal antibody

DHX58/LGP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11355-1-AP

Synonyms

D11LGP2, D11lgp2e, DHX58, DHX58/LGP2, LGP2, Protein D11Lgp2 homolog, RIG I like receptor LGP2, RLR



Tested Applications

Positive WB detected inHEK-293 cells, rat kidney tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human nephroblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11355-1-AP targets DHX58/LGP2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHX58/LGP2 fusion protein Ag1910
Full Name DEXH (Asp-Glu-X-His) box polypeptide 58
Calculated molecular weight 678 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC014949
Gene symbol DHX58
Gene ID (NCBI) 79132
RRIDAB_2092319
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHX58(Probable ATP-dependent RNA helicase DHX58) is also named as D11LGP2E, LGP2 and belongs to the RLR subfamily. DHX58, originally identified as a highly expressed gene in mammary tumors, is another cytoplasmic DEX(D/H)-box helicase that can recognize RNA(PMID: 18411269). It acts as a positive, but not negative, regulator of RIG-I-and MDA5-dependent recognition of RNA virus infection and plays a pivotal role in antiviral responses in vivo(PMID:20080593).

Protocols

Product Specific Protocols
WB protocol for DHX58/LGP2 antibody 11355-1-APDownload protocol
IHC protocol for DHX58/LGP2 antibody 11355-1-APDownload protocol
IP protocol for DHX58/LGP2 antibody 11355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

RIG-I-like receptors direct inflammatory macrophage polarization against West Nile virus infection.

Authors - Amy E L Stone
  • KO Validated
humanWB

Nucleic Acids Res

miR-122 does not impact recognition of the HCV genome by innate sensors of RNA but rather protects the 5' end from the cellular pyrophosphatases, DOM3Z and DUSP11.

Authors - Yalena Amador-Cañizares
humanWB

EMBO Rep

RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling.

Authors - Jean-Patrick Parisien
human,pig,monkeyWB,IF,IP

J Virol

LGP2 Promotes Type I Interferon Production To Inhibit PRRSV Infection via Enhancing MDA5-Mediated Signaling

Authors - Zhenbang Zhu
  • KD Validated
human,mouseWB

J Virol

Ambivalent role of the innate immune response in rabies virus pathogenesis.

Authors - Chopy Damien D
human,mouseWB,IHC,IP

Cancer Sci

Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.

Authors - Li-Ling Lin