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  • KD/KO Validated

LGP2 Polyclonal antibody

LGP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11355-1-AP

Synonyms

D11LGP2, D11lgp2e, DHX58, LGP2, Protein D11Lgp2 homolog, RIG I like receptor LGP2, RLR



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11355-1-AP targets LGP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LGP2 fusion protein Ag1910
Full Name DEXH (Asp-Glu-X-His) box polypeptide 58
Calculated molecular weight 678 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC014949
Gene symbol DHX58
Gene ID (NCBI) 79132
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHX58(Probable ATP-dependent RNA helicase DHX58) is also named as D11LGP2E, LGP2 and belongs to the RLR subfamily. DHX58, originally identified as a highly expressed gene in mammary tumors, is another cytoplasmic DEX(D/H)-box helicase that can recognize RNA(PMID: 18411269). It acts as a positive, but not negative, regulator of RIG-I-and MDA5-dependent recognition of RNA virus infection and plays a pivotal role in antiviral responses in vivo(PMID:20080593).

Protocols

Product Specific Protocols
WB protocol for LGP2 antibody 11355-1-APDownload protocol
IHC protocol for LGP2 antibody 11355-1-APDownload protocol
IP protocol for LGP2 antibody 11355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biochem Biophys Res Commun

DExH-Box helicase 58 enhances osteoblast differentiation of osteoblastic cells via Wnt/β-Catenin signaling.

Authors - Lei Zhou
  • KD Validated
humanWB

Biochem Biophys Res Commun

The TAR-RNA binding protein is required for immunoresponses triggered by Cardiovirus infection.

Authors - Akihiko Komuro
mouseWB

J Neurovirol

Characterization of retinoic acid-inducible gene-I expression in primary murine glia following exposure to vesicular stomatitis virus.

Authors - Furr Samantha R SR
human,mouseWB,IHC,IP

Cancer Sci

Innate immune sensor laboratory of genetics and physiology 2 suppresses tumor cell growth and functions as a prognostic marker in neuroblastoma.

Authors - Li-Ling Lin
human,mouseWB

J Virol

Ambivalent role of the innate immune response in rabies virus pathogenesis.

Authors - Chopy Damien D
humanWB

EMBO Rep

RNA sensor LGP2 inhibits TRAF ubiquitin ligase to negatively regulate innate immune signaling.

Authors - Jean-Patrick Parisien