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  • Western Blot (WB) analysis of various lysates using DIAPH2 Polyclonal antibody (31304-1-AP)

    WB analysis using 31304-1-AP (same clone as 31304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31304-1-AP (DIAPH2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31304-1-PBS in a different storage buffer formulation.

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Product Information

31304-1-PBS targets DIAPH2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DIAPH2 fusion protein Ag35280 Predict reactive species
Full Name diaphanous homolog 2 (Drosophila)
Calculated Molecular Weight126kDa
Observed Molecular Weight126-130 kDa
GenBank Accession NumberNM_006729.4
Gene Symbol DIAPH2
Gene ID (NCBI) 1730
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO60879
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.

Background Information

In vertebrates, three DRF genes (DIAPH1, DIAPH2 and DIAPH3) are the orthologs of the Drosophila melanogaster diaphanous (dia) gene, which has important roles in fertility and hearing in the fly. DIAPH1 and DIAPH3 have been associated with different types of hearing loss (HL). DIAPH2, located on chromosome Xq21.33, until now it has only been linked to premature ovarian failure 2 (POF2A). (PMID: 36689403)