DISC1-long-specific Polyclonal antibody

DISC1-long-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15500-1-AP

Synonyms

C1orf136, DISC1, DISC1 long, disrupted in schizophrenia 1, KIAA0457, SCZD9



Tested Applications

Positive WB detected inSH-SY5Y cells, human kidney tissue, mouse brain tissue, mouse kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15500-1-AP targets DISC1-long-specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name disrupted in schizophrenia 1
Calculated molecular weight 95 kDa
Observed molecular weight 60 kDa, 62 kDa, 75- 85 kDa
GenBank accession numberNM_018662
Gene symbol DISC1
Gene ID (NCBI) 27185
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DISC1, also named as KIAA0457, is identified as the sole gene whose ORF is truncated and cosegregates with major mental illnesses in a Scottish family. DISC1 may be implicated in psychiatric conditions in other populations than the unique Scottish family. It is involved in the regulation of multiple aspects of embryonic and adult neurogenesis. DISC1 is required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. It plays a role, together with PCNT, in the microtubule network formation. In WB, DISC1 has some MW such as 34kd(PMID:18620078 );75-85kd and 95-100kd(PMID:15657124). DISC1 isoforms have distinct developmental patterns of expression. This antibody is against C-terminal of DISC1, binding full length protein specifically.

Protocols

Product Specific Protocols
WB protocol for DISC1-long-specific antibody 15500-1-APDownload protocol
IHC protocol for DISC1-long-specific antibody 15500-1-APDownload protocol
IF protocol for DISC1-long-specific antibody 15500-1-APDownload protocol
IP protocol for DISC1-long-specific antibody 15500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Cell Neurosci

Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1.

Authors - Anna Sancho-Balsells