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  • KD/KO Validated

DKK1 Polyclonal antibody

DKK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 21112-1-AP


Dickkopf 1, Dickkopf related protein 1, DKK 1, DKK1, hDkk 1, SK

Tested Applications

Positive WB detected inA549 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman gliomas tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21112-1-AP is DKK1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DKK1 fusion protein Ag15110
Full Name dickkopf homolog 1 (Xenopus laevis)
Calculated Molecular Weight 266 aa, 29 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC001539
Gene Symbol DKK1
Gene ID (NCBI) 22943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kDa doublet protein, which is larger than the deduced molecular mass of 26 kDa. Sometime DKK1 is expressed as a 42- to 50-kDa secreted protein, with little change observed after glycanase treatment.


Product Specific Protocols
WB protocol for DKK1 antibody 21112-1-APDownload protocol
IHC protocol for DKK1 antibody 21112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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