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  • KD/KO Validated

DKK1 Polyclonal antibody

DKK1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 21112-1-AP

Synonyms

Dickkopf 1, Dickkopf related protein 1, DKK 1, DKK1, hDkk 1, SK



Tested Applications

Positive WB detected inA549 cells, K-562 cells
Positive IHC detected inhuman gliomas tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21112-1-AP is DKK1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rabbit, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DKK1 fusion protein Ag15110
Full Name dickkopf homolog 1 (Xenopus laevis)
Calculated molecular weight 266 aa, 29 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC001539
Gene symbol DKK1
Gene ID (NCBI) 22943
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kD doublet protein, which is larger than the deduced molecular mass of 26 kD. Sometime DKK1 is expressed as a 42- to 50-kD secreted protein, with little change observed after glycanase treatment.

Protocols

Product Specific Protocols
WB protocol for DKK1 antibody 21112-1-APDownload protocol
IHC protocol for DKK1 antibody 21112-1-APDownload protocol
IF protocol for DKK1 antibody 21112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Theranostics

BPI-9016M, a c-Met inhibitor, suppresses tumor cell growth, migration and invasion of lung adenocarcinoma via miR203-DKK1.

Authors - Panpan Zhang
  • KD Validated
humanWB

In Vitro Cell Dev Biol Anim

A1CF-Axin2 signal axis regulates apoptosis and migration in Wilms tumor-derived cells through Wnt/β-catenin pathway.

Authors - Dongsheng Ni
humanWB

J Exp Clin Cancer Res

Depleting PTOV1 sensitizes non-small cell lung cancer cells to chemotherapy through attenuating cancer stem cell traits.

Authors - Zhiqiang Wu
ratWB

Osteoporos Int

Rehmanniae Radix Preparata suppresses bone loss and increases bone strength through interfering with canonical Wnt/β-catenin signaling pathway in OVX rats.

Authors - C Liu

J Mol Histol

Distinctive role of ACVR1 in dentin formation: requirement for dentin thickness in molars and prevention of osteodentin formation in incisors of mice.

Authors - Xue Zhang
rabbitWB

Bone

Transcriptome sequencing profiling identifies miRNA-331-3p as an osteoblast-specific miRNA in infected bone nonunion.

Authors - Yang Zhang