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  • KD/KO Validated

Dystrophin Polyclonal antibody

Dystrophin Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12715-1-AP


BMD, CMD3B, DMD, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, dystrophin

Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue, mouse lung, rat brain
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue, rat heart tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12715-1-AP targets Dystrophin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dystrophin fusion protein Ag3408
Full Name dystrophin
Calculated Molecular Weight 3685 aa, 427 kDa
Observed Molecular Weight70 kDa, 430 kDa
GenBank Accession NumberBC028720
Gene Symbol DMD
Gene ID (NCBI) 1756
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.


Product Specific Protocols
WB protocol for Dystrophin antibody 12715-1-APDownload protocol
IHC protocol for Dystrophin antibody 12715-1-APDownload protocol
IF protocol for Dystrophin antibody 12715-1-APDownload protocol
IP protocol for Dystrophin antibody 12715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Biomed Eng

Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids.

Authors - Ningning Shi

Sci Adv

Semirational bioengineering of AAV vectors with increased potency and specificity for systemic gene therapy of muscle disorders

Authors - Jihad El Andari

Nat Commun

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Authors - I-Ling Lu
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Knockdown of Salusin-β Improves Cardiovascular Function in Myocardial Infarction-Induced Chronic Heart Failure Rats.

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Muscle cytotoxicity and immuno-reactivity analysis of the porous carbon nanospheres fabricated by high temperature calcination

Authors - JingWen Huang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Lauren (Verified Customer) (08-10-2018)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: stem cell-derived myotubes
Dystrophin Antibody Immunofluorescence, validation (1:100 dilution) in stem cell-derived myotubes (Cat no:12715-1-AP)