Validation Data Gallery
|Positive WB detected in||mouse brain tissue, mouse lung, rat brain|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human skeletal muscle tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse skeletal muscle tissue, rat heart tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12715-1-AP targets Dystrophin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Dystrophin fusion protein Ag3408|
|Calculated molecular weight||3685 aa, 427 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC028720|
|Gene ID (NCBI)||1756|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.
|Product Specific Protocols|
|WB protocol for Dystrophin antibody 12715-1-AP||Download protocol|
|IHC protocol for Dystrophin antibody 12715-1-AP||Download protocol|
|IF protocol for Dystrophin antibody 12715-1-AP||Download protocol|
|IP protocol for Dystrophin antibody 12715-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Lauren (Verified Customer) (08-10-2018)