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  • KD/KO Validated

Dystrophin Polyclonal antibody

Dystrophin Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12715-1-AP

Synonyms

BMD, CMD3B, DMD, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, dystrophin



Tested Applications

Positive WB detected inmouse brain tissue, mouse lung, rat brain
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12715-1-AP targets Dystrophin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dystrophin fusion protein Ag3408
Full Name dystrophin
Calculated molecular weight 3685 aa, 427 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC028720
Gene symbol DMD
Gene ID (NCBI) 1756
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix. Multiple isoforms of dystrophin exist due to the alternative splicing, with a wide range of MW (69-72, 110-143, 271, 426 kDa). Most tissues contain transcripts of several isoforms.

Protocols

Product Specific Protocols
WB protocol for Dystrophin antibody 12715-1-APDownload protocol
IHC protocol for Dystrophin antibody 12715-1-APDownload protocol
IF protocol for Dystrophin antibody 12715-1-APDownload protocol
IP protocol for Dystrophin antibody 12715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIP

Biochem Biophys Res Commun

Dp71 is regulated by phosphorylation and ubiquitin-proteasome system in neuronal cells.

Authors - Takahiro Fujimoto
humanIHC

Drug Des Devel Ther

Protective Effects of Rocuronium Bromide on Ischemia-Reperfusion Injury in Skeletal Muscle Induced by Tourniquet in Patients Undergoing Elective Unilateral Total Knee Arthroplasty: A Prospective, Double Blind, Randomized, Controlled Study.

Authors - Hui Chen
ratIF

Am J Physiol Endocrinol Metab

The exocyst complex regulates insulin-stimulated glucose uptake of skeletal muscle cells

Authors - Brent A Fujimoto
mouseIF

J Cell Mol Med

FGF21 promotes ischaemic angiogenesis and endothelial progenitor cells function under diabetic conditions in an AMPK/NAD+-dependent manner.

Authors - Qiaoxia Dai
humanWB,IP,IF

Hum Mol Genet

Dystroglycan regulates proper expression, submembranous localisation and subsequent phosphorylation of Dp71 through physical interaction.

Authors - Takahiro Fujimoto
humanWB

J Neuroinflammation

New insights into meningitic Escherichia coli infection of brain microvascular endothelial cells from quantitative proteomics analysis.

Authors - Wen-Tong Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lauren (Verified Customer) (08-10-2018)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: stem cell-derived myotubes
Dystrophin Antibody Immunofluorescence, validation (1:100 dilution) in stem cell-derived myotubes (Cat no:12715-1-AP)