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DNA-RNA Hybrid antibody (mAb) (Clone S9.6)

Host / Isotype

Mouse / IgG2a, k

Reactivity

Wide Range Predicted

Applications

ChIP, ChIP-Seq, DB, EMSA, ICC, IF, IP

CloneNo.

S9.6

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Cat No : 65683,65684 65683

Synonyms

dna-rna, monoclonal antibody, r-loops

Validation Data Gallery

  • DNA-RNA Hybrid antibody tested by dot blot. 23-mer DNA, RNA or DNA-RNA Hybrid samples were spotted (indicated in ng on the left) on to a positively charged nylon membrane and blotted with DNA-RNA Hybrid antibody at 1 ug/ml dilution. Lane 1: Double-stranded DNA-RNA hybrid. Lane 2; Single-stranded DNA oligo. Lane 3: SIngle-stranded RNA oligo.

    DNA-RNA Hybrid antibody tested by dot blot. 23-mer DNA, RNA or DNA-RNA Hybrid samples were spotted (indicated in ng on the left) on to a positively charged nylon membrane and blotted with DNA-RNA Hybrid antibody at 1 ug/ml dilution. Lane 1: Double-stranded DNA-RNA hybrid. Lane 2; Single-stranded DNA oligo. Lane 3: SIngle-stranded RNA oligo.

Product Information

Tested Applications ChIP, ChIP-Seq, DB, EMSA, ICC, IF, IP

Applications Validated by Active Motif: DB: 1 ug/ml.
Tested Reactivity Wide Range Predicted
Host / Isotype Mouse / IgG2a, k
Class Monoclonal
Type Antibody
Immunogen
Full Name DNA-RNA Hybrid antibody (mAb) (Clone S9.6)
Synonyms dna-rna, monoclonal antibody, r-loops
Molecular weight N/A
GenBank accession numberN/A
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

DNA-RNA hybrids are a natural occurrence within eukaryotic cells, with levels of these hybrids increasing at sites with high transcriptional activity, such as during transcription initiation, repression, and elongation. Because RNA-DNA hybrids influence genomic instability, the S9.6 antibody is a useful reagent to help study the consequences of R-loops and lesions formed by these hybrids during DNA replication or other cellular processes. In addition, the S9.6 antibody is effective in recognizing RNA-DNA hybridization for microarray studies.