|Positive WB detected in||mouse lung tissue, HepG2 cells, human liver tissue, human testis tissue, Jurkat cells, mouse kidney tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11713-1-AP targets DNAJA1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DNAJA1 fusion protein Ag2284|
|Full Name||DnaJ (Hsp40) homolog, subfamily A, member 1|
|Calculated molecular weight||397 aa, 45 kDa|
|Observed molecular weight||45 kDa|
|GenBank accession number||BC008182|
|Gene ID (NCBI)||3301|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.
J Biol Chem
A cytosolic chaperone complex controls folding and degradation of type III CD38.
Mol Biol Cell
DjA1 maintains Golgi integrity via interaction with GRASP65.
Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation.