DNAJA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNAJA1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF

Conjugate

Unconjugated

Publications

5

Cat no : 11713-1-AP

Synonyms

DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2

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Validation Data Gallery

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  WB analysis of mouse lung using 11713-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11713-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11713-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11713-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11713-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11713-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11713-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11713-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11713-1-AP (DNAJA1 antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of HepG2 using 11713-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11713-1-AP (DNAJA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse lung tissue, HepG2 cells, human liver tissue, human testis tissue, Jurkat cells, mouse kidney tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below

Product Information

11713-1-AP targets DNAJA1 in WB, IHC, IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJA1 fusion protein Ag2284
• Full Name: DnaJ (Hsp40) homolog, subfamily A, member 1
• Calculated molecular weight: 397 aa, 45 kDa
• Observed molecular weight: 45 kDa
• GenBank accession number: BC008182
• Gene symbol: DNAJA1
• Gene ID (NCBI): 3301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

Publications

Species	Application	Title
human	WB	J Biol Chem; A cytosolic chaperone complex controls folding and degradation of type III CD38.; Authors - Yang Wu
human	WB	Mol Biol Cell; DjA1 maintains Golgi integrity via interaction with GRASP65.; Authors - Jie Li ; KD Validated
human	WB	Sci Rep; Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation.; Authors - Claudio Rodríguez-González ; KO Validated
mouse	WB	Mol Ther Nucleic Acids; Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction; Authors - Jie Pan
human	WB	J Clin Invest; Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.; Authors - Yajuan Zhang ; KD Validated