DNAJA1 Polyclonal antibody

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  WB analysis using 11713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11713-1-AP

  WB result of DNAJA1 antibody (11713-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells.

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  WB analysis of human testis using 11713-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11713-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11713-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 11713-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11713-1-AP (DNAJA1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11713-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAJA1 antibody (11713-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

DNAJA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11713-1-AP

Publications(13)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

hDj-2, HDJ2, hDJ 2, DNAJ2, DjA1

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human testis tissue, HepG2 cells, human liver tissue, Jurkat cells, mouse lung tissue, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 12 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

11713-1-AP targets DNAJA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJA1 fusion protein Ag2284
• Full Name: DnaJ (Hsp40) homolog, subfamily A, member 1
• Calculated Molecular Weight: 397 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC008182
• Gene Symbol: DNAJA1
• Gene ID (NCBI): 3301
• RRID: AB_10637266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P31689
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

Publications

Species	Application	Title
human	WB,IF	Nature; A cytosolic surveillance mechanism activates the mitochondrial UPR; Authors - F X Reymond Sutandy ; KD Validated
human	IF	Nat Cell Biol; Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition; Authors - Shan Lu
human	WB,IP	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang
human	WB	J Clin Invest; Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.; Authors - Yajuan Zhang ; KD Validated
mouse	WB	Mol Ther Nucleic Acids; Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction; Authors - Jie Pan
human	WB	Sci Rep; Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation.; Authors - Claudio Rodríguez-González ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Filip (Verified Customer) (10-28-2024)

I recommend this antibody. It does not recognize DNAJA2, which we run in parallel to DNAJA1.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000