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DNAJA1 Polyclonal antibody
DNAJA1 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF
Conjugate
Unconjugated
Cat no : 11713-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse lung tissue, HepG2 cells, human liver tissue, human testis tissue, Jurkat cells, mouse kidney tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 5 publications below |
Product Information
11713-1-AP targets DNAJA1 in WB, IHC, IF applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DNAJA1 fusion protein Ag2284 |
Full Name | DnaJ (Hsp40) homolog, subfamily A, member 1 |
Calculated molecular weight | 397 aa, 45 kDa |
Observed molecular weight | 45 kDa |
GenBank accession number | BC008182 |
Gene symbol | DNAJA1 |
Gene ID (NCBI) | 3301 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.
Protocols
Product Specific Protocols | |
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WB protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
IHC protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
IF protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Chem A cytosolic chaperone complex controls folding and degradation of type III CD38. | ||
Sci Rep Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation.
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Mol Ther Nucleic Acids Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction | ||
J Clin Invest Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.
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