• Featured Product
  • KD/KO Validated

DNAJA1 Polyclonal antibody

DNAJA1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11713-1-AP

Synonyms

DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2



Tested Applications

Positive WB detected inmouse lung tissue, HepG2 cells, human liver tissue, human testis tissue, Jurkat cells, mouse kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11713-1-AP targets DNAJA1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJA1 fusion protein Ag2284
Full Name DnaJ (Hsp40) homolog, subfamily A, member 1
Calculated molecular weight 397 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC008182
Gene symbol DNAJA1
Gene ID (NCBI) 3301
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

Protocols

Product Specific Protocols
WB protocol for DNAJA1 antibody 11713-1-APDownload protocol
IHC protocol for DNAJA1 antibody 11713-1-APDownload protocol
IF protocol for DNAJA1 antibody 11713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

A cytosolic chaperone complex controls folding and degradation of type III CD38.

Authors - Yang Wu
humanWB

Mol Biol Cell

DjA1 maintains Golgi integrity via interaction with GRASP65.

Authors - Jie Li
  • KD Validated
humanWB

Sci Rep

Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation.

Authors - Claudio Rodríguez-González
  • KO Validated