DNLZ Polyclonal antibody

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  WB analysis using 31844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31844-1-AP (DNLZ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat heart using 31844-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 31844-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 31844-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 31844-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31844-1-AP (DNLZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 31844-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DNLZ antibody (31844-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

DNLZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31844-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

mtHsp70-escort protein, Hsp70-escort protein 1, HEP1, DNL-type zinc finger protein, C9orf151

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Tested Applications

• Positive WB detected in: RT-4 cells
• Positive IHC detected in: mouse heart tissue, rat heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31844-1-AP targets DNLZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNLZ fusion protein Ag37114
• Full Name: DNL-type zinc finger
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC146915
• Gene Symbol: DNLZ
• Gene ID (NCBI): 728489
• RRID: AB_3670126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5SXM8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNL-type zinc finger (DNLZ, also known as HEP, ZIM17, TIMM15, and C9orf151) is a nuclear-encoded protein that is translocated to the mitochondria, thus it is located in mitochondrion and nucleoplasm (PMID: 22162012). It may function as a co-chaperone towards HSPA9/mortalin, which enhances HSPA9 solubility and stimulates HSPA9 catalytic activity (PMID: 18632665; 20392697; 21530495).