DRP1 (C-terminal) Polyclonal antibody

• Featured Product
• KD/KO Validated

DRP1 (C-terminal) Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IP, IF, FC, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

189

Reviews (3)

Cat no : 12957-1-AP

Synonyms

DLP1, DNM1L, DRP1, DRP1 (C-terminal), DVLP, dynamin 1 like, Dynamin 1 like protein, Dynamin like protein, Dynamin like protein 4, Dynamin related protein 1

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Validation Data Gallery

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  WB analysis using 12957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12957-1-AP

  WB result of DRP1 (C-terminal) antibody (12957-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRP1 (C-terminal) transfected HeLa cells.

• 

  IP experiment of mouse brain using 12957-1-AP

  IP result of anti-DRP1 (C-terminal) (IP:12957-1-AP, 4ug; Detection:12957-1-AP 1:1000) with mouse brain tissue lysate 2400 ug.

• 

  IHC staining of mouse cerebellum using 12957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12957-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 12957-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DRP1 (C-terminal) (12957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, A549 cells, rat brain tissue, DU 145 cells, HCT 116 cells, HepG2 cells, NIH/3T3 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 182 publications below
• IHC: See 12 publications below
• IF: See 18 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

12957-1-AP targets DRP1 (C-terminal) in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, monkey, zebrafish, hamster, duck, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DRP1 (C-terminal) fusion protein Ag3644
• Full Name: dynamin 1-like
• Calculated Molecular Weight: 736 aa, 82 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC024590
• Gene Symbol: DNM1L
• Gene ID (NCBI): 10059
• RRID: AB_2093525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB,IHC,IP	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
human	WB	Cell Metab; Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.; Authors - Jing Yuan
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
chicken	WB,IF	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (03-06-2023)

It works pretty well in human white adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes

FH

Samrajni (Verified Customer) (04-27-2022)

The product works well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 cell line

FH

Tom (Verified Customer) (07-07-2021)

Paraformaldehyde fixation and Triton X-100 permeabilization.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa

DRP1 (C-terminal) Antibody Immunofluorescence validation (1:100 dilution) in HeLa (Cat no:12957-1-AP)

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