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  • KD/KO Validated

DRP1 (C-terminal) Polyclonal antibody

DRP1 (C-terminal) Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (6)





Cat no : 12957-1-AP


DLP1, DNM1L, DRP1, DRP1 (C-terminal), DVLP, dynamin 1 like, Dynamin 1 like protein, Dynamin like protein, Dynamin like protein 4, Dynamin related protein 1

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, A549 cells, rat brain tissue, DU 145 cells, HCT 116 cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12957-1-AP targets DRP1 (C-terminal) in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey, zebrafish, hamster, duck, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DRP1 (C-terminal) fusion protein Ag3644
Full Name dynamin 1-like
Calculated Molecular Weight 736 aa, 82 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC024590
Gene Symbol DNM1L
Gene ID (NCBI) 10059
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.


Product Specific Protocols
WB protocol for DRP1 (C-terminal) antibody 12957-1-APDownload protocol
IHC protocol for DRP1 (C-terminal) antibody 12957-1-APDownload protocol
IF protocol for DRP1 (C-terminal) antibody 12957-1-APDownload protocol
IP protocol for DRP1 (C-terminal) antibody 12957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Cell Metab

Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.

Authors - Jing Yuan

Protein Cell

Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice

Authors - He-Zhou Huang

Bioact Mater

Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis

Authors - Wei Zhang
  • KD Validated

ACS Cent Sci

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Authors - Juan Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Mi (Verified Customer) (03-06-2023)

It works pretty well in human white adipocyte cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: adipocytes

Samrajni (Verified Customer) (04-27-2022)

The product works well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12 cell line

Tom (Verified Customer) (07-07-2021)

Paraformaldehyde fixation and Triton X-100 permeabilization.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HeLa
DRP1 (C-terminal) Antibody Immunofluorescence validation (1:100 dilution) in HeLa (Cat no:12957-1-AP)