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DRP1 (C-terminal) Polyclonal antibody
DRP1 (C-terminal) Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 12957-1-AP
Validation Data Gallery
|Positive WB detected in||mouse brain tissue, HeLa cells, A549 cells, rat brain tissue, DU 145 cells, HCT 116 cells, HepG2 cells, NIH/3T3 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12957-1-AP targets DRP1 (C-terminal) in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, monkey, zebrafish, hamster, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DRP1 (C-terminal) fusion protein Ag3644|
|Full Name||dynamin 1-like|
|Calculated molecular weight||736 aa, 82 kDa|
|Observed molecular weight||75-80 kDa|
|GenBank accession number||BC024590|
|Gene ID (NCBI)||10059|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.
|Product Specific Protocols|
|WB protocol for DRP1 (C-terminal) antibody 12957-1-AP||Download protocol|
|IHC protocol for DRP1 (C-terminal) antibody 12957-1-AP||Download protocol|
|IF protocol for DRP1 (C-terminal) antibody 12957-1-AP||Download protocol|
|IP protocol for DRP1 (C-terminal) antibody 12957-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Mi (Verified Customer) (03-06-2023)
It works pretty well in human white adipocyte cells.
Samrajni (Verified Customer) (04-27-2022)
The product works well.
Tom (Verified Customer) (07-07-2021)
Paraformaldehyde fixation and Triton X-100 permeabilization.