DRP1 (C-terminal) Polyclonal antibody

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  WB analysis using 12957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12957-1-AP

  WB result of DRP1 (C-terminal) antibody (12957-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRP1 (C-terminal) transfected HeLa cells.

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  IP experiment of mouse brain using 12957-1-AP

  IP result of anti-DRP1 (C-terminal) (IP:12957-1-AP, 4ug; Detection:12957-1-AP 1:1000) with mouse brain tissue lysate 2400 ug.

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  IHC staining of mouse cerebellum using 12957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 12957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12957-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DRP1 (C-terminal) antibody (12957-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 12957-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DRP1 (C-terminal) (12957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

DRP1 (C-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12957-1-AP

Publications(286)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

DRP1, Dymple, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, A549 cells, rat brain tissue, DU 145 cells, HCT 116 cells, HepG2 cells, NIH/3T3 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 272 publications below
• IHC: See 22 publications below
• IF: See 30 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

12957-1-AP targets DRP1 (C-terminal) in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, chicken, zebrafish, bovine, hamster, goat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DRP1 (C-terminal) fusion protein Ag3644
• Full Name: dynamin 1-like
• Calculated Molecular Weight: 736 aa, 82 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC024590
• Gene Symbol: DRP1
• Gene ID (NCBI): 10059
• RRID: AB_2093525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00429
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
human	WB,IHC,IP	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
mouse	WB	Cell Stem Cell; Mechano-oncogenic cytoskeletal remodeling drives leukemic transformation with mitochondrial vesicle-mediated STING activation; Authors - Zemin Song ; KO Validated
human	WB	Cell Metab; Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.; Authors - Jing Yuan
mouse	WB	Protein Cell; Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice; Authors - He-Zhou Huang
mouse	WB,IF	Bioact Mater; Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis; Authors - Wei Zhang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

P (Verified Customer) (09-23-2024)

Very Good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEK

DRP1 (C-terminal) Antibody Western Blot validation (1000 dilution) in HEK (Cat no:12957-1-AP)

FH

Mi (Verified Customer) (03-06-2023)

It works pretty well in human white adipocyte cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes

FH

Samrajni (Verified Customer) (04-27-2022)

The product works well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12 cell line

FH

Tom (Verified Customer) (07-07-2021)

Paraformaldehyde fixation and Triton X-100 permeabilization.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa

DRP1 (C-terminal) Antibody Immunofluorescence validation (1:100 dilution) in HeLa (Cat no:12957-1-AP)