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  • KD/KO Validated

DRD1 Polyclonal antibody

DRD1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17934-1-AP

Synonyms

D(1A) dopamine receptor, DADR, Dopamine D1 receptor, dopamine receptor D1, DRD1, DRD1A



Tested Applications

Positive WB detected in37°C incubated mouse brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17934-1-AP targets DRD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DRD1 fusion protein Ag12366
Full Name dopamine receptor D1
Calculated Molecular Weight 446 aa, 49 kDa
Observed Molecular Weight 50-75 kDa
GenBank Accession NumberBC074978
Gene Symbol DRD1
Gene ID (NCBI) 1812
RRIDAB_10598308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dopamine is a neurotransmitter that plays a crucial role in physical and mental health, such as cardiovascular, hormonal, renal and central nervous systems (PMID: 29220802). Five subtypes of mammalian dopamine receptors are grouped into two classes, the D1- and D2-like classes. The D1-like class includes D1 and D5 receptors whereas the D2-like class includes D2, D3, D4 subtypes (PMID: 9457173). Dopamine receptor D1 (DRD1) is the most abundant form of dopamine receptor in the central nervous system. DRD1 stimulates adenylate cyclase, modulates D2 receptor activity, regulates neuron growth and differentiation, and mediates several behavioral responses (PMID: 1977312). DRD1 has a calculated molecular weight of 49 kDa, larger apparent molecular weight of 60-80 kDa may be due to glycosylation (PMID: 1281547; 23821371).

Protocols

Product Specific Protocols
WB protocol for DRD1 antibody 17934-1-APDownload protocol
IHC protocol for DRD1 antibody 17934-1-APDownload protocol
IF protocol for DRD1 antibody 17934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

PMID: 25594175

Authors - Yiqing Yan
  • KD Validated
ratWB

EMBO Mol Med

MicroRNA expression profile and functional analysis reveal that miR-382 is a critical novel gene of alcohol addiction.

Authors - Li Jingyuan J
humanIF

Elife

The organic cation transporter 2 regulates dopamine D1 receptor signaling at the Golgi apparatus.

Authors - Natasha M Puri
ratWB

Int J Mol Sci

Effects of Maternal High-Fructose Diet on Long Non-Coding RNAs and Anxiety-like Behaviors in Offspring

Authors - Yuchen Zou
mouseIF

CNS Neurosci Ther

Amphiregulin blockade decreases the levodopa-induced dyskinesia in a 6-hydroxydopamine Parkinson's disease mouse model

Authors - Piniel Alphayo Kambey
humanWB

Mol Neurobiol

Protective Effect of Curcumin by Modulating BDNF/DARPP32/CREB in Arsenic-Induced Alterations in Dopaminergic Signaling in Rat Corpus Striatum.

Authors - Pranay Srivastava