Desmocollin 2 Polyclonal antibody

Desmocollin 2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13876-1-AP

Synonyms

ARVD11, Cadherin family member 2, CDHF2, desmocollin 2, Desmocollin 3, Desmosomal glycoprotein II, Desmosomal glycoprotein III, DG2, DGII/III, DSC2, DSC3



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman oesophagus tissue, human skin cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

13876-1-AP targets Desmocollin 2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Desmocollin 2 fusion protein Ag4956
Full Name desmocollin 2
Calculated molecular weight 100 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC063291
Gene symbol DSC2
Gene ID (NCBI) 1824
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Desmocollin 2 (DSC2) is a member of the desmocollin subfamily of the cadherin superfamily. The desmocollins (DSC1-3), along with the desmogleins (DSG1-4), are found primarily in epithelia and cardiac muscle where they constitute the adhesive proteins of the intercellular desmosome junctions and are required for cell adhesion and desmosome formation. Mutations in DSC2 gene are associated with arrhythmogenic right ventricular dysplasia-11.

Protocols

Product Specific Protocols
WB protocol for Desmocollin 2 antibody 13876-1-APDownload protocol
IHC protocol for Desmocollin 2 antibody 13876-1-APDownload protocol
IF protocol for Desmocollin 2 antibody 13876-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Dev Cell

Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model.

Authors - Sally Dabelsteen