DSG2 Polyclonal antibody

DSG2 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 21880-1-AP

Synonyms

ARVC10, ARVD10, Cadherin family member 5, CDHF5, desmoglein 2, DSG2, HDGC



Tested Applications

Positive WB detected inHepG2 cells, A431 cells, A549 cells, HEK-293 cells, HeLa cells
Positive IP detected inHepG2 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21880-1-AP is DSG2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DSG2 fusion protein Ag16393
Full Name desmoglein 2
Calculated molecular weight 1118 aa, 122 kDa
Observed molecular weight 145-150 kDa
GenBank accession numberBC099655
Gene symbol DSG2
Gene ID (NCBI) 1829
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).

Protocols

Product Specific Protocols
WB protocol for DSG2 antibody 21880-1-APDownload protocol
IF protocol for DSG2 antibody 21880-1-APDownload protocol
IP protocol for DSG2 antibody 21880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Helicobacter

Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.

Authors - Chen Zhang
humanWB

Virol Sin

Desmoglein 2 (DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia.

Authors - Jing Zhang
  • KD Validated
mouseWB

Food Chem Toxicol

Sodium fluoride and sulfur dioxide affected male reproduction by disturbing blood-testis barrier in mice.

Authors - Jianhai Zhang
humanIF

Mol Biol Cell

Plakophilin 3 and Par3 Facilitate Desmosomes' association with the Apical Junctional Complex.

Authors - Indrajyoti Indra
humanIF

EMBO Rep

O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.

Authors - Ieva Bagdonaite