Validation Data Gallery
|Positive WB detected in
|HepG2 cells, HEK-293 cells, A431 cells, A549 cells, HeLa cells
|Positive IP detected in
|Positive IHC detected in
|human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:500-1:2000
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
21880-1-AP targets DSG2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|DSG2 fusion protein Ag16393
|Calculated molecular weight
|1118 aa, 122 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).
|Product Specific Protocols
|WB protocol for DSG2 antibody 21880-1-AP
|IHC protocol for DSG2 antibody 21880-1-AP
|IF protocol for DSG2 antibody 21880-1-AP
|IP protocol for DSG2 antibody 21880-1-AP
|Click here to view our Standard Protocols
O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.
Desmoglein 2 (DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia.
Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.
Mol Biol Cell
Plakophilin 3 and Par3 Facilitate Desmosomes' association with the Apical Junctional Complex.
Food Chem Toxicol
Sodium fluoride and sulfur dioxide affected male reproduction by disturbing blood-testis barrier in mice.
Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Saba (Verified Customer) (09-26-2022)
it is said that this antibody is rabbit, however, unfortunately we observed a significant cross reaction with secondary anti mouse antibody.