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  • KD/KO Validated

DSG2 Polyclonal antibody

DSG2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21880-1-AP

Synonyms

ARVC10, ARVD10, Cadherin family member 5, CDHF5, desmoglein 2, DSG2, HDGC



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, A431 cells, A549 cells, HeLa cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

21880-1-AP targets DSG2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DSG2 fusion protein Ag16393
Full Name desmoglein 2
Calculated molecular weight 1118 aa, 122 kDa
Observed molecular weight 145-150 kDa
GenBank accession numberBC099655
Gene symbol DSG2
Gene ID (NCBI) 1829
RRIDAB_10836933
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).

Protocols

Product Specific Protocols
WB protocol for DSG2 antibody 21880-1-APDownload protocol
IHC protocol for DSG2 antibody 21880-1-APDownload protocol
IF protocol for DSG2 antibody 21880-1-APDownload protocol
IP protocol for DSG2 antibody 21880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

EMBO Rep

O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.

Authors - Ieva Bagdonaite
humanWB

Virol Sin

Desmoglein 2 (DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia.

Authors - Jing Zhang
  • KD Validated
humanIF

Helicobacter

Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.

Authors - Chen Zhang
humanIF

Mol Biol Cell

Plakophilin 3 and Par3 Facilitate Desmosomes' association with the Apical Junctional Complex.

Authors - Indrajyoti Indra
mouseWB

Food Chem Toxicol

Sodium fluoride and sulfur dioxide affected male reproduction by disturbing blood-testis barrier in mice.

Authors - Jianhai Zhang
mouseWB

Microscopy (Oxf)

Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.

Authors - Shoko Ishikawa

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Saba (Verified Customer) (09-26-2022)

it is said that this antibody is rabbit, however, unfortunately we observed a significant cross reaction with secondary anti mouse antibody.

  • Applications: Immunofluorescence