Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lung cancer tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21880-1-AP targets DSG2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16393 Product name: Recombinant human DSG2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 819-1118 aa of BC099655 Sequence: GELDDRFLDDLGLKFKTLAEVCLGQKIDINKEIEQRQKPATETSMNTASHSLCEQTMVNSENTYSSGSSFPVPKSLQEANAEKVTQEIVTERSVSSRQAQKVATPLPDPMASRNVIATETSYVTGSTMPPTTVILGPSQPQSLIVTERVYAPASTLVDQPYANEGTVVVTERVIQPHGGGSNPLEGTQHLQDVPYVMVRERESFLAPSSGVQPTLAMPNIAVGQNVTVTERVLAPASTLQSSYQIPTENSMTARNTTVSGAGVPGPLPDFGLEESGHSNSTITTSSTRVTKHSTVQHSYS Predict reactive species |
| Full Name | desmoglein 2 |
| Calculated Molecular Weight | 1118 aa, 122 kDa |
| Observed Molecular Weight | 145-150 kDa |
| GenBank Accession Number | BC099655 |
| Gene Symbol | DSG2 |
| Gene ID (NCBI) | 1829 |
| RRID | AB_10836933 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14126 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DSG2 antibody 21880-1-AP | Download protocol |
| IHC protocol for DSG2 antibody 21880-1-AP | Download protocol |
| IP protocol for DSG2 antibody 21880-1-AP | Download protocol |
| WB protocol for DSG2 antibody 21880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DSG2 Polyclonal antibody (Cat# 21880-1-AP) has 18 citations published in journals including Cell, Nature Communications, EMBO Reports, Journal of Cell Biology, Molecular Biology of the Cell, BMC Cancer, and others. Research fields span cell adhesion and desmosome biology, cancer (breast, oral, cervical), epithelial differentiation, aging/proteomics, infectious disease (adenovirus, H. pylori), reproductive biology, and keratinocyte function. Applications include WB, IF, IHC, and CoIP across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Cell Comprehensive human proteome profiles across a 50-year lifespan reveal aging trajectories and signatures | ||
Nat Commun Actomyosin forces trigger a conformational change in desmoplakin within desmosomes. | ||
Nat Commun Extracellular matrix mediates circulating tumor cell clustering in triple-negative breast cancer metastasis. | ||
Int J Biol Macromol Colon-targeted chitosan-gold nanoparticles loaded with Pueraria flavonoids for ulcerative colitis therapy via barrier repair and immune modulation.
| ||
J Cell Biol Two δ-catenins, plakophilin 4 and p120, promote formation of distinct types of adherens junctions. | ||
EMBO Rep O-glycan initiation directs distinct biological pathways and controls epithelial differentiation. |
Reviews
What customers say
Customer reviews for this DSG2 antibody are mixed. One user reported a clear, well-localized signal at the intercalated disc in iPSC-derived cardiomyocytes using immunofluorescence at 1:400 dilution, rating it 4 out of 5 stars. Another user gave 1 star, noting significant cross-reaction with a secondary anti-mouse antibody despite the primary being rabbit-derived, which caused unexpected background in their IF experiment. Overall, the antibody can produce specific staining when properly matched with the correct secondary, but users should be cautious about potential cross-reactivity issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kiara (Verified Customer) (07-28-2025) | This DSG2 primary antibody gave us a nice signal that is located at the intercalated disc as expected.
 |
Saba (Verified Customer) (09-26-2022) | it is said that this antibody is rabbit, however, unfortunately we observed a significant cross reaction with secondary anti mouse antibody.
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