Validation Data Gallery
|Positive WB detected in||HepG2 cells, HEK-293 cells, HeLa cells, A431 cells, A549 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21880-1-AP is DSG2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DSG2 fusion protein Ag16393|
|Full Name||desmoglein 2|
|Calculated molecular weight||1118 aa, 122 kDa|
|Observed molecular weight||145-150 kDa|
|GenBank accession number||BC099655|
|Gene ID (NCBI)||1829|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).
|Product Specific Protocols|
|WB protocol for DSG2 antibody 21880-1-AP||Download protocol|
|IHC protocol for DSG2 antibody 21880-1-AP||Download protocol|
|IF protocol for DSG2 antibody 21880-1-AP||Download protocol|
|IP protocol for DSG2 antibody 21880-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.
Desmoglein 2 (DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia.
Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.
Food Chem Toxicol
Sodium fluoride and sulfur dioxide affected male reproduction by disturbing blood-testis barrier in mice.
Mol Biol Cell
Plakophilin 3 and Par3 Facilitate Desmosomes' association with the Apical Junctional Complex.
O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.