Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, HeLa cells, HepG2 cells
|Western Blot (WB)
|WB : 1:500-1:1000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
55001-1-AP targets Drosha in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|ribonuclease type III, nuclear
|Calculated molecular weight
|Observed molecular weight
|90 kDa, 151 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA. DROSHA/RNASEN has some transcription forms with MW 160kd, 151kd, 138kd and 90kd.
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