E2F4 Polyclonal antibody

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E2F4 Polyclonal Antibody for WB, IP, ELISA

Cat No. 10923-1-AP Copied!

Publications (24)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Synonyms

E2F 4, E2F-4, Transcription factor E2F4

• 

  WB analysis of HeLa using 10923-1-AP

  WB result of E2F4 antibody (10923-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F4 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 10923-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 10923-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 10923-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HL-60 using 10923-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 10923-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 10923-1-AP

  IP result of anti-E2F4 (IP:10923-1-AP, 3ug; Detection:10923-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells, HL-60 cells, Raji cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10923-1-AP targets E2F4 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1365

  Product name: Recombinant human E2F4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-413 aa of BC033180

  Sequence: MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQWKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVPPPEDLLQSPSAVSTPPPLPKPALAQSQEASRPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSNSNSSSSSGPNPSTSFEPIKADPTGVLELPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLNL

• Full Name: E2F transcription factor 4, p107/p130-binding
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC033180
• Gene Symbol: E2F4
• Gene ID (NCBI): 1874
• RRID: AB_2097096
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16254
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.

Publications

What published studies show

The E2F4 Polyclonal antibody (Cat# 10923-1-AP) has 24 citations. These appear in journals including Signal Transduction and Targeted Therapy, Cancer Research, Nature Communications, Protein & Cell, Advanced Science, Oncogene, Cell Reports, Cell Death & Disease, Frontiers in Immunology, and PLoS One, among others. Research fields span cancer biology (breast, colorectal, esophageal, ovarian, and liver cancers), cell cycle and senescence regulation, stem cell differentiation, inflammation, epigenetics, and viral infection. Applications include WB, IHC, IF, IP, and ChIP.

Automatically generated from the 24 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Signal Transduct Target Ther; Family with sequence similarity 114 member A1 orchestrates immune evasion in triple-negative breast cancer; Authors - Wenhao Zhang
human	WB,IHC,ChIP	Cancer Res; METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion.; Authors - Xiaowei She ; KD Validated
mouse	WB,ChIP	Protein Cell; Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis.; Authors - Yuting Wang
human	WB,IP,IF,ChIP	Nat Commun; Trim33 masks a non-transcriptional function of E2f4 in replication fork progression; Authors - Vanessa Rousseau
mouse	WB	J Adv Res; Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling; Authors - Di Fan
mouse	WB	Adv Sci (Weinh); TTK Inhibition Alleviates Postinjury Neointimal Formation and Atherosclerosis; Authors - Jie-Hong Wu