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E2F4 Polyclonal antibody

E2F4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10923-1-AP

Synonyms

E2F 4, E2F4, Transcription factor E2F4



Tested Applications

Positive WB detected inA431 cells, Jurkat cells, HL-60 cells, Raji cells, HeLa cells, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10923-1-AP targets E2F4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E2F4 fusion protein Ag1365
Full Name E2F transcription factor 4, p107/p130-binding
Calculated molecular weight 44 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC033180
Gene symbol E2F4
Gene ID (NCBI) 1874
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.

Protocols

Product Specific Protocols
WB protocol for E2F4 antibody 10923-1-APDownload protocol
IHC protocol for E2F4 antibody 10923-1-APDownload protocol
IP protocol for E2F4 antibody 10923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Amino Acid Deprivation-Induced Autophagy Requires Upregulation of DIRAS3 through Reduction of E2F1 and E2F4 Transcriptional Repression.

Authors - Margie N Sutton
humanWB

PLoS One

Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells.

Authors - Valle Blanca L BL
mouseWB,IHC

J Cell Physiol

Stable overexpression of p130/E2F4 affects the multipotential abilities of bone-marrow-derived mesenchymal stem cells.

Authors - Xiwen Zhang
humanWB

Oncogene

Regulatory networks in mechanotransduction reveal key genes in promoting cancer cell stemness and proliferation.

Authors - Wei Huang