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E2F4 Polyclonal antibody
E2F4 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ChIP
Conjugate
Unconjugated
Cat no : 10923-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, Jurkat cells, HL-60 cells, Raji cells, HeLa cells, NIH/3T3 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
ChIP | See 1 publications below |
Product Information
10923-1-AP targets E2F4 in WB, IP, IHC, ChIP applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | E2F4 fusion protein Ag1365 |
Full Name | E2F transcription factor 4, p107/p130-binding |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 60-70 kDa |
GenBank accession number | BC033180 |
Gene symbol | E2F4 |
Gene ID (NCBI) | 1874 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.
Protocols
Product Specific Protocols | |
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WB protocol for E2F4 antibody 10923-1-AP | Download protocol |
IHC protocol for E2F4 antibody 10923-1-AP | Download protocol |
IP protocol for E2F4 antibody 10923-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Protein Cell Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis. | ||
Oncogene Regulatory networks in mechanotransduction reveal key genes in promoting cancer cell stemness and proliferation. | ||
J Cell Physiol Stable overexpression of p130/E2F4 affects the multipotential abilities of bone-marrow-derived mesenchymal stem cells. | ||
PLoS One Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells. | ||
Cell Death Dis Metformin sensitises hepatocarcinoma cells to methotrexate by targeting dihydrofolate reductase.
| ||
Cancers (Basel) Amino Acid Deprivation-Induced Autophagy Requires Upregulation of DIRAS3 through Reduction of E2F1 and E2F4 Transcriptional Repression. |