E2F4 Polyclonal antibody

• Featured Product
• KD/KO Validated

E2F4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ChIP

Conjugate

Unconjugated

Publications

6

Cat no : 10923-1-AP

Synonyms

E2F 4, E2F4, Transcription factor E2F4

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Validation Data Gallery

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  WB analysis of HeLa using 10923-1-AP

  WB result of E2F4 antibody (10923-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F4 transfected HeLa cells.

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  WB analysis of A431 using 10923-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10923-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10923-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10923-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10923-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10923-1-AP

  IP Result of anti-E2F4 (IP:10923-1-AP, 3ug; Detection:10923-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

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  IHC staining of human lymphoma using 10923-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10923-1-AP (E2F4 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells, HL-60 cells, Raji cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

10923-1-AP targets E2F4 in WB, IP, IHC, ChIP applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: E2F4 fusion protein Ag1365
• Full Name: E2F transcription factor 4, p107/p130-binding
• Calculated molecular weight: 44 kDa
• Observed molecular weight: 60-70 kDa
• GenBank accession number: BC033180
• Gene symbol: E2F4
• Gene ID (NCBI): 1874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.

Publications

Species	Application	Title
mouse	WB,ChIP	Protein Cell; Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis.; Authors - Yuting Wang
human	WB	Oncogene; Regulatory networks in mechanotransduction reveal key genes in promoting cancer cell stemness and proliferation.; Authors - Wei Huang
mouse	WB,IHC	J Cell Physiol; Stable overexpression of p130/E2F4 affects the multipotential abilities of bone-marrow-derived mesenchymal stem cells.; Authors - Xiwen Zhang
human	WB	PLoS One; Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells.; Authors - Valle Blanca L BL
human	WB	Cell Death Dis; Metformin sensitises hepatocarcinoma cells to methotrexate by targeting dihydrofolate reductase.; Authors - Yinghui Wang ; KD Validated
human	WB	Cancers (Basel); Amino Acid Deprivation-Induced Autophagy Requires Upregulation of DIRAS3 through Reduction of E2F1 and E2F4 Transcriptional Repression.; Authors - Margie N Sutton