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  • KD/KO Validated

E2F4 Polyclonal antibody

E2F4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10923-1-AP


E2F 4, E2F4, Transcription factor E2F4

Tested Applications

Positive WB detected inA431 cells, Jurkat cells, HL-60 cells, Raji cells, HeLa cells, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10923-1-AP targets E2F4 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E2F4 fusion protein Ag1365
Full Name E2F transcription factor 4, p107/p130-binding
Calculated molecular weight 44 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC033180
Gene symbol E2F4
Gene ID (NCBI) 1874
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.


Product Specific Protocols
WB protocol for E2F4 antibody 10923-1-APDownload protocol
IHC protocol for E2F4 antibody 10923-1-APDownload protocol
IP protocol for E2F4 antibody 10923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Protein Cell

Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis.

Authors - Yuting Wang


Regulatory networks in mechanotransduction reveal key genes in promoting cancer cell stemness and proliferation.

Authors - Wei Huang

J Cell Physiol

Stable overexpression of p130/E2F4 affects the multipotential abilities of bone-marrow-derived mesenchymal stem cells.

Authors - Xiwen Zhang

PLoS One

Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells.

Authors - Valle Blanca L BL

Cell Death Dis

Metformin sensitises hepatocarcinoma cells to methotrexate by targeting dihydrofolate reductase.

Authors - Yinghui Wang
  • KD Validated

Cancers (Basel)

Amino Acid Deprivation-Induced Autophagy Requires Upregulation of DIRAS3 through Reduction of E2F1 and E2F4 Transcriptional Repression.

Authors - Margie N Sutton