EDNRB Polyclonal antibody
EDNRB Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA, IF
Conjugate
Unconjugated
Cat no : 20964-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse placenta tissue, human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 1 publications below |
| FC | See 1 publications below |
Product Information
20964-1-AP targets EDNRB in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | endothelin receptor type B |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 53-60 kDa |
| GenBank Accession Number | NM_000115 |
| Gene Symbol | EDNRB |
| Gene ID (NCBI) | 1910 |
| RRID | AB_2878774 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in ships and can be bound by EDN1. Still, while EDNRA mediates a constrictive effect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EDNRB antibody 20964-1-AP | Download protocol |
| IHC protocol for EDNRB antibody 20964-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Nucleic acid ligands act as a PAM and agonist depending on the intrinsic ligand binding state of P2RY2. | ||
Vascul Pharmacol Tail vein injection of mmLDL upregulates mouse mesenteric artery ETB receptors via activation of the ERK1/2 pathway. | ||
Pigment Cell Melanoma Res The immunosuppressive role of Edn3 overexpression in the melanoma microenvironment. | ||
Open Med (Wars) EDNRB inhibits the growth and migration of prostate cancer cells by activating the cGMP-PKG pathway | ||
J Pharm Pharmacol Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Jaouhara (Verified Customer) (12-05-2023) | Immunofluorescence analysis of paraffin-fixed murine chondrocytes using anti-EDNRB (20964-1-AP) and an Alexa 647 secondary antibody. Also validated in flow cytometry and immunohistochemistry.
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