EDNRB Polyclonal antibody
EDNRB Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
WB, IHC, IF, FC, ELISA
Cat no : 20964-1-AP
|Positive WB detected in||HEK-293 cells, mouse placenta tissue, human placenta tissue|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|WB||See 4 publications below|
|IF||See 1 publications below|
|FC||See 1 publications below|
20964-1-AP targets EDNRB in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||endothelin receptor type B|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||53 kDa|
|GenBank accession number||NM_000115|
|Gene ID (NCBI)||1910|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in vessels and can be bound by EDN1, but while EDNRA mediates a constrictive effffect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849).
|Product Specific Protocols|
|WB protocol for EDNRB antibody 20964-1-AP||Download protocol|
|IHC protocol for EDNRB antibody 20964-1-AP||Download protocol|
|FC protocol for EDNRB antibody 20964-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Proc Natl Acad Sci U S A
Nucleic acid ligands act as a PAM and agonist depending on the intrinsic ligand binding state of P2RY2.
Tail vein injection of mmLDL upregulates mouse mesenteric artery ETB receptors via activation of the ERK1/2 pathway.
Pigment Cell Melanoma Res
The immunosuppressive role of Edn3 overexpression in the melanoma microenvironment.
Functions of retinal astrocytes and Müller cells in mammalian myopia
J Pharm Pharmacol
Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway.