EDNRB Polyclonal antibody

EDNRB Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 20964-1-AP

Synonyms

ABCDS, EDNRB, Endothelin B receptor, endothelin receptor type B, ET B, ET BR, ETB, ETBR, ETRB, HSCR, HSCR2



Tested Applications

Positive WB detected inmouse placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

20964-1-AP targets EDNRB in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name endothelin receptor type B
Calculated molecular weight 50 kDa
Observed molecular weight 53 kDa
GenBank accession numberNM_000115
Gene symbol EDNRB
Gene ID (NCBI) 1910
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EDNRB antibody 20964-1-APDownload protocol
IHC protocol for EDNRB antibody 20964-1-APDownload protocol
FC protocol for EDNRB antibody 20964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Pharm Pharmacol

Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway.

Authors - Zhong-San Zeng
mouseWB

Vascul Pharmacol

Tail vein injection of mmLDL upregulates mouse mesenteric artery ETB receptors via activation of the ERK1/2 pathway.

Authors - Yang Liu