EDNRB Polyclonal antibody

EDNRB Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20964-1-AP

Synonyms

ABCDS, EDNRB, Endothelin B receptor, endothelin receptor type B, ET B, ET BR, ETB, ETBR, ETRB, HSCR, HSCR2



Tested Applications

Positive WB detected inHEK-293 cells, mouse placenta tissue, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20964-1-AP targets EDNRB in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name endothelin receptor type B
Calculated molecular weight 50 kDa
Observed molecular weight 53 kDa
GenBank accession numberNM_000115
Gene symbol EDNRB
Gene ID (NCBI) 1910
RRIDAB_2878774
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in vessels and can be bound by EDN1, but while EDNRA mediates a constrictive effect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849).

Protocols

Product Specific Protocols
WB protocol for EDNRB antibody 20964-1-APDownload protocol
IHC protocol for EDNRB antibody 20964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Nucleic acid ligands act as a PAM and agonist depending on the intrinsic ligand binding state of P2RY2.

Authors - Masaki Takahashi
mouseWB

Vascul Pharmacol

Tail vein injection of mmLDL upregulates mouse mesenteric artery ETB receptors via activation of the ERK1/2 pathway.

Authors - Yang Liu
mouseIF,FC

Pigment Cell Melanoma Res

The immunosuppressive role of Edn3 overexpression in the melanoma microenvironment.

Authors - Juliano T Freitas
mouseWB

BMC Ophthalmol

Functions of retinal astrocytes and Müller cells in mammalian myopia

Authors - Xuhong Zhang
mouseWB

J Pharm Pharmacol

Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway.

Authors - Zhong-San Zeng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jaouhara (Verified Customer) (12-05-2023)

Immunofluorescence analysis of paraffin-fixed murine chondrocytes using anti-EDNRB (20964-1-AP) and an Alexa 647 secondary antibody. Also validated in flow cytometry and immunohistochemistry.

  • Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry
  • Cell Tissue Type: Chondrocytes
EDNRB Antibody Immunohistochemistry,Immunofluorescence,Flow Cytometry validation ( dilution) in Chondrocytes (Cat no:20964-1-AP)