EEF1B2 Polyclonal antibody

• Featured Product
• KD/KO Validated

EEF1B2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (1)

Cat no : 10483-1-AP

Synonyms

EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta

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Validation Data Gallery

• 

  WB analysis of HeLa using 10483-1-AP

  WB result of EEF1B2 antibody (10483-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells.

• 

  WB analysis of PC-3 using 10483-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10483-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10483-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10483-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10483-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10483-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10483-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10483-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10483-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10483-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10483-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10483-1-AP (EEF1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10483-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10483-1-AP (EEF1B2 antibody) at dilution of 1:100 (under 25x lens).

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Tested Applications

• Positive WB detected in: PC-3 cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, SKOV-3 cells, mouse kidney tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

10483-1-AP targets EEF1B2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EEF1B2 fusion protein Ag0742
• Full Name: eukaryotic translation elongation factor 1 beta 2
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC004931
• Gene Symbol: EEF1B2
• Gene ID (NCBI): 1933
• RRID: AB_2096985
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against full length EEF1B2 of human origin.

Publications

Species	Application	Title
human	WB	PLoS Genet; Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.; Authors - Emily M Schleicher ; KD Validated
human	WB,IF,IP	Mol Cell Biol; Translational repression by RNA-binding protein TIAR.; Authors - Mazan-Mamczarz Krystyna K
mouse	WB	Front Mol Biosci; Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.; Authors - Taku Kaitsuka
rat	WB	Proteomics; Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver.; Authors - Fofana Bourlaye B
human	WB	Amino Acids; Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA.; Authors - Yusuke Terui
human,mouse	WB	PLoS One; Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.; Authors - Pitteri Sharon J SJ

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (01-17-2020)

KO validated by KO mice

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart