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  • KD/KO Validated

EEF1B2 Polyclonal antibody

EEF1B2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10483-1-AP


EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta

Tested Applications

Positive WB detected inPC-3 cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, SKOV-3 cells, mouse kidney tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10483-1-AP targets EEF1B2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF1B2 fusion protein Ag0742
Full Name eukaryotic translation elongation factor 1 beta 2
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC004931
Gene Symbol EEF1B2
Gene ID (NCBI) 1933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against full length EEF1B2 of human origin.


Product Specific Protocols
WB protocol for EEF1B2 antibody 10483-1-APDownload protocol
IHC protocol for EEF1B2 antibody 10483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



PLoS Genet

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

Authors - Emily M Schleicher
  • KD Validated

Mol Cell Biol

Translational repression by RNA-binding protein TIAR.

Authors - Mazan-Mamczarz Krystyna K

Front Mol Biosci

Heat Shock-Induced Dephosphorylation of Eukaryotic Elongation Factor 1BδL by Protein Phosphatase 1.

Authors - Taku Kaitsuka


Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver.

Authors - Fofana Bourlaye B

Amino Acids

Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA.

Authors - Yusuke Terui

PLoS One

Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.

Authors - Pitteri Sharon J SJ


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


WEI (Verified Customer) (01-17-2020)

KO validated by KO mice

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart