EEF1B2 Polyclonal antibody

EEF1B2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10483-1-AP

Synonyms

EEF1B, EEF1B1, EEF1B2, EF 1 beta, EF1B, Elongation factor 1 beta



Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse kidney tissue, SKOV-3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10483-1-AP targets EEF1B2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF1B2 fusion protein Ag0742
Full Name eukaryotic translation elongation factor 1 beta 2
Calculated molecular weight 29 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC004931
Gene symbol EEF1B2
Gene ID (NCBI) 1933
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against full length EEF1B2 of human origin.

Protocols

Product Specific Protocols
WB protocol for EEF1B2 antibody 10483-1-APDownload protocol
IHC protocol for EEF1B2 antibody 10483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Mol Med

Deficiency of TPPP2, a factor linked to oligoasthenozoospermia, causes subfertility in male mice.

Authors - Feng Zhu
humanWB

PLoS Genet

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

Authors - Emily M Schleicher
  • KD Validated
humanWB

Biotechniques

En masse analysis of nascent translation using microarrays.

Authors - Mazan-Mamczarz Krystyna K
human,mouseWB

PLoS One

Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.

Authors - Pitteri Sharon J SJ
humanWB

Amino Acids

Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA.

Authors - Yusuke Terui
ratWB

Proteomics

Prenatal alcohol exposure alters phosphorylation and glycosylation of proteins in rat offspring liver.

Authors - Fofana Bourlaye B

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

WEI (Verified Customer) (01-17-2020)

KO validated by KO mice

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart