EFEMP2 Polyclonal antibody

EFEMP2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12004-1-AP

Synonyms

EFEMP2, FBLN4, FIBL 4, Fibulin 4, MBP1, Protein UPH1, UPH1



Tested Applications

Positive WB detected inmouse kidney tissue, K-562 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman heart tissue, human prostate cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

12004-1-AP targets EFEMP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EFEMP2 fusion protein Ag2640
Full Name EGF-containing fibulin-like extracellular matrix protein 2
Calculated molecular weight 443 aa, 49 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC010456
Gene symbol EFEMP2
Gene ID (NCBI) 30008
RRIDAB_10667002
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EFEMP2 antibody 12004-1-APDownload protocol
IHC protocol for EFEMP2 antibody 12004-1-APDownload protocol
IP protocol for EFEMP2 antibody 12004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Biol Ther

STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2

Authors - Dong Zhang
humanWB

Cell Mol Biol Lett

EFEMP2 upregulates PD-L1 expression via EGFR/ERK1/2/c-Jun signaling to promote the invasion of ovarian cancer cells

Authors - Xin Shen