EFEMP2 Polyclonal antibody

EFEMP2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12004-1-AP

Synonyms

EFEMP2, FBLN4, FIBL 4, Fibulin 4, MBP1, Protein UPH1, UPH1

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Validation Data Gallery

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  WB analysis of mouse kidney using 12004-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12004-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12004-1-AP (EFEMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12004-1-AP

  IP Result of anti-EFEMP2 (IP:12004-1-AP, 4ug; Detection:12004-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12004-1-AP (EFEMP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 12004-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 12004-1-AP (EFEMP2 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, K-562 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human heart tissue, human prostate cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

12004-1-AP targets EFEMP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EFEMP2 fusion protein Ag2640
• Full Name: EGF-containing fibulin-like extracellular matrix protein 2
• Calculated molecular weight: 443 aa, 49 kDa
• Observed molecular weight: 52-55 kDa
• GenBank accession number: BC010456
• Gene symbol: EFEMP2
• Gene ID (NCBI): 30008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Biol Ther; STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2; Authors - Dong Zhang