EFEMP2 Polyclonal antibody
EFEMP2 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12004-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, K-562 cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human heart tissue, human prostate cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
Product Information
12004-1-AP targets EFEMP2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | EFEMP2 fusion protein Ag2640 |
Full Name | EGF-containing fibulin-like extracellular matrix protein 2 |
Calculated molecular weight | 443 aa, 49 kDa |
Observed molecular weight | 52-55 kDa |
GenBank accession number | BC010456 |
Gene symbol | EFEMP2 |
Gene ID (NCBI) | 30008 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
IHC protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
IP protocol for EFEMP2 antibody 12004-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Biol Ther STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2 |