EFTUD2 Polyclonal antibody

EFTUD2 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 10208-1-AP

Synonyms

EFTUD2, hSNU114, KIAA0031, Snrp116, Snu114, SNU114 homolog, U5 116 kDa, U5 116KD



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10208-1-AP targets EFTUD2 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EFTUD2 fusion protein Ag0279
Full Name elongation factor Tu GTP binding domain containing 2
Calculated molecular weight 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC002360
Gene symbol EFTUD2
Gene ID (NCBI) 9343
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EFTUD2 encodes the 116 kDa U5 small nuclear ribonecleoprotein (snRNP) component, also named SNU114, it has GTP binding activity and is required for pre-mRNA splicing. Evolutionarily conserved human snRNP protein (U5-116kDa) is homologous to the ribosomal elongation factor EF-2 (ribosomal translocase). Defects in EFTUD2 are the cause of mandibulofacial dysostosis with microcephaly (MFDM). So far, acetylation and phosphorylation sites of EFTUD2 have been identified. Catolog#10208-1-AP is a rabbit polyclonal antibody raised against the N-terminal of human EFTUD2.

Protocols

Product Specific Protocols
WB protocol for EFTUD2 antibody 10208-1-APDownload protocol
IF protocol for EFTUD2 antibody 10208-1-APDownload protocol
IP protocol for EFTUD2 antibody 10208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

Reduced prostaglandin I2 signaling in Arid5b-/- primary skeletal muscle cells attenuates myogenesis.

Authors - Jennifer Murray
humanWB

Proteomics Clin Appl

New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells.

Authors - Huang Rui R
mouseWB

Am J Physiol Cell Physiol

Estrogen-related receptor α regulates skeletal myocyte differentiation via modulation of the ERK MAP kinase pathway.

Authors - Murray Jennifer J
humanWB

J Mol Biol

Proteomic characterization of the nucleolar linker histone H1 interaction network.

Authors - Heather J Szerlong
humanWB

Proteomics

A Proteomic approach to discover and compare interacting partners of Papillomavirus E2 proteins from diverse phylogenetic groups.

Authors - Moon Kyoo Jang
humanWB,IP

J Biol Chem

Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.

Authors - Zhe Chen