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  • KD/KO Validated

EGFR Monoclonal antibody

EGFR Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2A2H10

Cat no : 66455-1-Ig

Synonyms

EGFR, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1



Tested Applications

Positive WB detected inHepG2 cells, A549 cells, SKOV-3 cells, PC-3 cells, A431 cells, HeLa cells, LNCaP cells, PC-3 clles
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue, human cervical cancer tissue, human colon cancer tissue, human gliomas tissue, human lung cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:20000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66455-1-Ig targets EGFR in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EGFR fusion protein Ag24947
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight 1210 aa, 134 kDa
Observed molecular weight 145-165 kDa
GenBank accession numberBC094761
Gene symbol EGFR
Gene ID (NCBI) 1956
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.

Protocols

Product Specific Protocols
WB protocol for EGFR antibody 66455-1-IgDownload protocol
IHC protocol for EGFR antibody 66455-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IHC

Cancers (Basel)

Honokiol Eliminates Glioma/Glioblastoma Stem Cell-Like Cells Via JAK-STAT3 Signaling and Inhibits Tumor Progression by Targeting Epidermal Growth Factor Receptor.

Authors - Yipu Fan
humanWB

Cell Rep

EPS8 Facilitates Uncoating of Influenza A Virus.

Authors - Gloria P Larson
mouseWB

Biomed Pharmacother

Serum proteomics analysis based on label-free revealed the protective effect of Chinese herbal formula Gu-Ben-Fang-Xiao.

Authors - Qiong-Qiong Xing
humanWB,IF

Talanta

Aptamer-based CRISPR/Cas12a assay for the ultrasensitive detection of extracellular vesicle proteins.

Authors - Huilan Li
humanWB,IHC

Cell Death Dis

MUC1 induces acquired chemoresistance by upregulating ABCB1 in EGFR-dependent manner.

Authors - Wei Jin
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other