|Positive WB detected in||HepG2 cells, A549 cells, SKOV-3 cells, PC-3 cells, A431 cells, HeLa cells, LNCaP cells, PC-3 clles|
|Positive IHC detected in||human tonsillitis tissue, human breast cancer tissue, human cervical cancer tissue, human colon cancer tissue, human gliomas tissue, human lung cancer tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:10000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
66455-1-Ig targets EGFR in WB, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||EGFR fusion protein Ag24947|
|Full Name||epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)|
|Calculated molecular weight||1210 aa, 134 kDa|
|Observed molecular weight||145-165 kDa|
|GenBank accession number||BC094761|
|Gene ID (NCBI)||1956|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.
Honokiol Eliminates Glioma/Glioblastoma Stem Cell-Like Cells Via JAK-STAT3 Signaling and Inhibits Tumor Progression by Targeting Epidermal Growth Factor Receptor.
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Serum proteomics analysis based on label-free revealed the protective effect of Chinese herbal formula Gu-Ben-Fang-Xiao.
Aptamer-based CRISPR/Cas12a assay for the ultrasensitive detection of extracellular vesicle proteins.
Dual signal amplification based on polysaccharide-initiated ring-opening polymerization and click polymerization for exosomes detection.
Cell Death Dis
MUC1 induces acquired chemoresistance by upregulating ABCB1 in EGFR-dependent manner.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Carly (Verified Customer) (11-17-2020)
Tested using EDTA plasma on an antibody microarray