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  • KD/KO Validated

PERK/EIF2AK3 Polyclonal antibody

PERK/EIF2AK3 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 24390-1-AP

Synonyms

EIF2AK3, PERK, Pancreatic eIF2 alpha kinase, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24390-1-AP targets PERK/EIF2AK3 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PERK/EIF2AK3 fusion protein Ag18254
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Calculated Molecular Weight 1116 aa, 125 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC126354
Gene Symbol PERK
Gene ID (NCBI) 9451
RRIDAB_2879521
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).

Protocols

Product Specific Protocols
WB protocol for PERK/EIF2AK3 antibody 24390-1-APDownload protocol
IHC protocol for PERK/EIF2AK3 antibody 24390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation.

Authors - Feixiang Yuan

J Adv Res

Postantibiotic leukocyte enhancement-mediated reduction of intracellular bacteria by macrophages

Authors - Yifan Wu
humanWB

Autophagy

Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.

Authors - Tengfei Huang
pigWB

Autophagy

STING1 is essential for an RNA-virus triggered autophagy.

Authors - Rui Zhang
  • KD Validated
pigWB

Autophagy

Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.

Authors - Sheng-Li Ming
mouseWB

Br J Pharmacol

Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation

Authors - Hang Su