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AGO2 Polyclonal antibody

AGO2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 10686-1-AP


AGO2, Argonaute2, eIF 2C 2, eIF2C 2, EIF2C2, hAgo2, PAZ Piwi domain protein, PPD, Protein argonaute 2, Protein slicer, Q10

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10686-1-AP targets AGO2 in WB, IP, RIP, CLIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, fish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGO2 fusion protein Ag1032
Full Name eukaryotic translation initiation factor 2C, 2
Calculated Molecular Weight 97 kDa
Observed Molecular Weight94-97 kDa
GenBank Accession NumberBC007633
Gene Symbol EIF2C2
Gene ID (NCBI) 27161
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions. This antibody is specific to EIF2C2. EIF2C2 exists two isoforms and molecular weight of isoforms are 97 kDa and 94 kDa.


Product Specific Protocols
WB protocol for AGO2 antibody 10686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Faezeh (Verified Customer) (01-29-2023)

Although it was hard to get, it shows the expectable result.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: A549 cell line
AGO2 Antibody Western Blot validation (1:1000 dilution) in A549 cell line (Cat no:10686-1-AP)