EIF3G Polyclonal antibody, PBS Only

• 

  WB analysis of HepG2 using 11165-1-AP (same clone as 11165-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11165-1-AP (EIF3G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 11165-1-AP (same clone as 11165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11165-1-AP (same clone as 11165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11165-1-AP (same clone as 11165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 11165-1-AP (same clone as 11165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 11165-1-AP (same clone as 11165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 11165-1-AP (same clone as 11165-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11165-1-AP (same clone as 11165-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 11165-1-AP (EIF3G antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 11165-1-AP (same clone as 11165-1-PBS)

  Immunofluorescent analysis of A549 cells, using EIF3G antibody 11165-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

EIF3G Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 11165-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

eIF-3 RNA-binding subunit, eIF3 p44, eIF3 p42, eIF3 delta, eIF 3 RNA binding subunit

---

Product Information

11165-1-PBS targets EIF3G in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF3G fusion protein Ag1653
• Full Name: eukaryotic translation initiation factor 3, subunit G
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC000733
• Gene Symbol: EIF3G
• Gene ID (NCBI): 8666
• RRID: AB_2096911
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75821
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Eukaryotic translation initiation factor eIF3, that plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. The EIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex. The complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3G is a component of the EIF3 complex. [PMID:20679478,20503360]