|Positive WB detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Sample-dependent, check data in validation data gallery|
11310-1-AP targets EIF3H in WB, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EIF3H fusion protein Ag1850|
|Full Name||eukaryotic translation initiation factor 3, subunit H|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||40 kDa|
|GenBank accession number||BC000386|
|Gene ID (NCBI)||8667|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Eukaryotic translation initiation factor eIF3, that plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. The EIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex. The complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3H is a component of the EIF3 complex. [PMID:19204574,20503360]
Biosci Biotechnol Biochem
The proliferation of colorectal cancer cells is suppressed by silencing of EIF3H.
Exp Ther Med
Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis.