EIF3H Polyclonal antibody

• 

  WB analysis using 11310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11310-1-AP (EIF3H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

EIF3H Polyclonal Antibody for WB, ELISA

Cat No. 11310-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Synonyms

eIF 3 gamma, eIF3 gamma, eIF3 p40, eIF3 p40 subunit, EIF3H, EIF3S3

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11310-1-AP targets EIF3H in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF3H fusion protein Ag1850
• Full Name: eukaryotic translation initiation factor 3, subunit H
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC000386
• Gene Symbol: EIF3H
• Gene ID (NCBI): 8667
• RRID: AB_2277723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15372
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation initiation factor eIF3, that plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. The EIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex. The complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3H is a component of the EIF3 complex. [PMID:19204574,20503360]

Publications

Species	Application	Title
human	WB	Stem Cell Res Ther; Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.; Authors - Mengqi Chu ; KD Validated
human	WB	Biosci Biotechnol Biochem; The proliferation of colorectal cancer cells is suppressed by silencing of EIF3H.; Authors - Genhua Yu ; KD Validated
human	WB	Exp Ther Med; Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis.; Authors - Song Hong ; KD Validated
human	WB	Nat Commun; METTL3 acetylation impedes cancer metastasis via fine-tuning its nuclear and cytosolic functions; Authors - Yuanpei Li
human	WB,IF,IP	Cell Commun Signal; The deubiquitinase EIF3H promotes hepatocellular carcinoma progression by stabilizing OGT and inhibiting ferroptosis; Authors - Jianing Tang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (11-01-2024)

great with ecl

• Applications: Western Blot