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  • KD/KO Validated

EIF3I Polyclonal antibody

EIF3I Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11287-1-AP

Synonyms

eIF 3 beta, eIF3 beta, eIF3 p36, EIF3I, EIF3S2, PRO2242, TRIP 1, TRIP1



Tested Applications

Positive WB detected inHEK-293 cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells, HeLa cells, MCF-7 cells, K-562 cells, human placenta tissue, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11287-1-AP targets EIF3I in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3I fusion protein Ag1847
Full Name eukaryotic translation initiation factor 3, subunit I
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000413
Gene symbol EIF3I
Gene ID (NCBI) 8668
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic initiation factor (EIF3) complex is the largest of the eIFs and consists of more than 10 nonidentical subunit, and is required for several steps in the initiation of protein synthesis. It associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). Also it can stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3I is one subunit of EIF3 complex.

Protocols

Product Specific Protocols
WB protocol for EIF3I antibody 11287-1-APDownload protocol
IHC protocol for EIF3I antibody 11287-1-APDownload protocol
IF protocol for EIF3I antibody 11287-1-APDownload protocol
IP protocol for EIF3I antibody 11287-1-APDownload protocol
FC protocol for EIF3I antibody 11287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem J

Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity.

Authors - Emma Jane Mead
humanWB,IF,CoIP

Ecotoxicol Environ Saf

Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway.

Authors - Yujing Zhang
mouse, hamsterWB

Biochem J

The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h.

Authors - Anne Roobol
ratIHC,WB

J Bone Miner Res

TRIP-1: a regulator of osteoblast function.

Authors - Metz-Estrella Diana D
  • KD Validated
humanWB

Metab Eng

Engineered transient and stable overexpression of translation factors eIF3i and eIF3c in CHOK1 and HEK293 cells gives enhanced cell growth associated with increased c-Myc expression and increased recombinant protein synthesis.

Authors - Anne Roobol