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EIF3I Polyclonal antibody
EIF3I Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11287-1-AP
|Positive WB detected in||HEK-293 cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells, HeLa cells, MCF-7 cells, K-562 cells, human placenta tissue, NIH/3T3 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue, human liver tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11287-1-AP targets EIF3I in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||hamster, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EIF3I fusion protein Ag1847|
|Full Name||eukaryotic translation initiation factor 3, subunit I|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC000413|
|Gene ID (NCBI)||8668|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Eukaryotic initiation factor (EIF3) complex is the largest of the eIFs and consists of more than 10 nonidentical subunit, and is required for several steps in the initiation of protein synthesis. It associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). Also it can stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3I is one subunit of EIF3 complex.
|Product Specific Protocols|
|WB protocol for EIF3I antibody 11287-1-AP||Download protocol|
|IHC protocol for EIF3I antibody 11287-1-AP||Download protocol|
|IF protocol for EIF3I antibody 11287-1-AP||Download protocol|
|IP protocol for EIF3I antibody 11287-1-AP||Download protocol|
|FC protocol for EIF3I antibody 11287-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity.
Ecotoxicol Environ Saf
Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway.
The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h.
J Bone Miner Res
TRIP-1: a regulator of osteoblast function.
Engineered transient and stable overexpression of translation factors eIF3i and eIF3c in CHOK1 and HEK293 cells gives enhanced cell growth associated with increased c-Myc expression and increased recombinant protein synthesis.