Tested Applications
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells, HeLa cells, MCF-7 cells, K-562 cells, human placenta tissue, NIH/3T3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue, human liver tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11287-1-AP targets EIF3I in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1847 Product name: Recombinant human EIF3I protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC000413 Sequence: MKPILLQGHERSITQIKYNREGDLLFTVAKDPIVNVWYSVNGERLGTYMGHTGAVWCVDADWDTKHVLTGSADNSCRLWDCETGKQLALLKTNSAVRTCGFDFGGNIIMFSTDKQMGYQCFVSFFDLRDPSQIDNNEPYMKIPCNDSKITSAVWGPLGECIIAGHESGELNQYSAKSGEVLVNVKEHSRQINDIQLSRDMTMFVTASKDNTAKLFDSTTLEHQKTFRTERPVNSAALSPNYDHVVLGGGQEAMDVTTTSTRIGKFEARFFHLAFEEEFGRVKGHFGPINSVAFHPDGKSYSSGGEDGYVRIHYFDPQYFEFEFEA Predict reactive species |
| Full Name | eukaryotic translation initiation factor 3, subunit I |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC000413 |
| Gene Symbol | EIF3I |
| Gene ID (NCBI) | 8668 |
| RRID | AB_2097068 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13347 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Eukaryotic initiation factor (EIF3) complex is the largest of the eIFs and consists of more than 10 nonidentical subunit, and is required for several steps in the initiation of protein synthesis. It associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). Also it can stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3I is one subunit of EIF3 complex.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF3I antibody 11287-1-AP | Download protocol |
| IHC protocol for EIF3I antibody 11287-1-AP | Download protocol |
| IP protocol for EIF3I antibody 11287-1-AP | Download protocol |
| WB protocol for EIF3I antibody 11287-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF3I Polyclonal antibody (Cat# 11287-1-AP) has 16 citations. Journals include Nucleic Acids Research, Advanced Science, British Journal of Cancer, Metabolic Engineering, Journal of Biological Chemistry, Biochemical Journal, Journal of Bone and Mineral Research, and others. Research fields span translation initiation regulation, cancer metastasis and survival, recombinant protein production, cellular senescence, bone biology, viral infection, RNA processing, tumor microenvironment remodeling, and cardiac ischemia/reperfusion injury.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res An interaction between eIF4A3 and eIF3g drives the internal initiation of translation | ||
Adv Sci (Weinh) eIF4E Enriched Extracellular Vesicles Induce Immunosuppressive Macrophages through HMGCR-Mediated Metabolic Rewiring | ||
Br J Cancer eIF3i facilitates NELFCD translation to promote metastasis via regulating EMT and invadopodia.
| ||
Metab Eng Engineered transient and stable overexpression of translation factors eIF3i and eIF3c in CHOK1 and HEK293 cells gives enhanced cell growth associated with increased c-Myc expression and increased recombinant protein synthesis. | ||
Ecotoxicol Environ Saf Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway. | ||
Cell Biosci METTL3 drives NSCLC metastasis by enhancing CYP19A1 translation and oestrogen synthesis |
Reviews
What customers say
The single review for this product reports a positive experience. The antibody performed well for Western Blot on HEK293 cells, used at a 1:2000 dilution. The reviewer gave it a 5-star rating, indicating high satisfaction with the product's performance in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fabienne (Verified Customer) (06-26-2023) | the antibody works well for Western Blot
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