EIF3J Antibody 10439-1-AP

Tested Applications

Applications:

ELISA, WB, IP, IHC, IF
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HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP(EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Immunohistochemical of paraffin-embedded human breast cancer using 10439-1-AP(EIF3J antibody) at dilution of 1:100 (under 10x lens)

Immunofluorescent analysis of Hela cells, using EIF3J antibody 10439-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

IP Result of anti-EIF3J (IP:10439-1-AP, 4ug; Detection:10439-1-AP 1:500) with HeLa cells lysate 880ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

HeLa cells, A549 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, SKOV-3 cells

Positive IP detected in:

HeLa cells

Positive IHC detected in:

human breast cancer tissue

Positive IF detected in:

Hela cells

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:20-1:200

IF : 1:20-1:200

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

EIF3J fusion protein ag0678

Full name:

eukaryotic translation initiation factor 3, subunit J

Calculated molecular weight:

28 kDa

Observed molecular weight:

35 kDa

GenBank accession number:

BC002719

Gene ID (NCBI):

8669

Gene symbol

EIF3J

Synonyms

eIF 3 alpha, eIF3 alpha, eIF3 p35, EIF3J, EIF3S1

Background

EIF3 has a key role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex(PMID:17588516). The eIF3 complex stimulates several steps in the translation initiation pathway, including dissociation of 80S ribosomes into 40S and 60S subunits, binding of a ternary complex (TC) consisting of Met-tRNA , eIF2, and GTP to the small subunit (forming the 43S preinitiation complex) and recruitment of mRNA to the 43 S complex to produce the 48S complex (PMID:11560931) EIF3J was identified as a high copy suppressor of the temperature-sensitive (Ts-) phenotype of the rpg1-1 allele of TIF32/RPG1, encoding the largest subunit of yeast eIF3 (eIF3a) (PMID:10488093).

Showing 1- [1] of [1] Publications

Author:		Ramírez-Valle Francisco F
Pubmed ID:		18426977
Journal:		J Cell Biol
Title:		eIF4GI links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy.
Application:		WB,IF,IP
KD validated
Species:		human

WB Figures

A549 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP(EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP(EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP(EIF3J antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP(EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 10439-1-AP(EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP(EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP(EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP(EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human breast cancer using 10439-1-AP(EIF3J antibody) at dilution of 1:10 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using 10439-1-AP(EIF3J antibody) at dilution of 1:100 (under 10x lens)

IP Figures

IP Result of anti-EIF3J (IP:10439-1-AP, 4ug; Detection:10439-1-AP 1:500) with HeLa cells lysate 880ug.

IF Figures

Immunofluorescent analysis of Hela cells, using EIF3J antibody 10439-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

EIF3J Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 10439-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [1] of [1] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

EIF3J Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 10439-1-AP

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Tested Applications

Product Information

Immunogen Information

Background