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EIF3J Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 10439-1-AP

Featured Product KD/KO validated

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Con: 33 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

EIF3J Antibody WB HeLa cells 10439-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP(EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

EIF3J Antibody IHC human breast cancer tissue 10439-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10439-1-AP(EIF3J antibody) at dilution of 1:100 (under 10x lens)

EIF3J Antibody IF Hela cells 10439-1-AP

Immunofluorescent analysis of Hela cells, using EIF3J antibody 10439-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

EIF3J Antibody IP HeLa cells 10439-1-AP

IP Result of anti-EIF3J (IP:10439-1-AP, 4ug; Detection:10439-1-AP 1:500) with HeLa cells lysate 880ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells, SKOV-3 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
EIF3J fusion protein Ag0678
Full name:
eukaryotic translation initiation factor 3, subunit J
Calculated molecular weight:
28 kDa
Observed molecular weight:
35 kDa
GenBank accession number:
BC002719
Gene ID (NCBI):
8669
Gene symbol
EIF3J
Synonyms
eIF 3 alpha, eIF3 alpha, eIF3 p35, EIF3J, EIF3S1

Background

EIF3 has a key role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex(PMID:17588516). The eIF3 complex stimulates several steps in the translation initiation pathway, including dissociation of 80S ribosomes into 40S and 60S subunits, binding of a ternary complex (TC) consisting of Met-tRNA , eIF2, and GTP to the small subunit (forming the 43S preinitiation complex) and recruitment of mRNA to the 43 S complex to produce the 48S complex (PMID:11560931) EIF3J was identified as a high copy suppressor of the temperature-sensitive (Ts-) phenotype of the rpg1-1 allele of TIF32/RPG1, encoding the largest subunit of yeast eIF3 (eIF3a) (PMID:10488093).


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