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EIF3J Polyclonal antibody

EIF3J Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10439-1-AP

Synonyms

eIF 3 alpha, eIF3 alpha, eIF3 p35, EIF3J, EIF3S1



Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells, SKOV-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10439-1-AP targets EIF3J in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3J fusion protein Ag0678
Full Name eukaryotic translation initiation factor 3, subunit J
Calculated molecular weight 28 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002719
Gene symbol EIF3J
Gene ID (NCBI) 8669
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF3 has a key role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex(PMID:17588516). The eIF3 complex stimulates several steps in the translation initiation pathway, including dissociation of 80S ribosomes into 40S and 60S subunits, binding of a ternary complex (TC) consisting of Met-tRNA , eIF2, and GTP to the small subunit (forming the 43S preinitiation complex) and recruitment of mRNA to the 43 S complex to produce the 48S complex (PMID:11560931) EIF3J was identified as a high copy suppressor of the temperature-sensitive (Ts-) phenotype of the rpg1-1 allele of TIF32/RPG1, encoding the largest subunit of yeast eIF3 (eIF3a) (PMID:10488093).

Protocols

Product Specific Protocols
WB protocol for EIF3J antibody 10439-1-APDownload protocol
IHC protocol for EIF3J antibody 10439-1-APDownload protocol
IF protocol for EIF3J antibody 10439-1-APDownload protocol
IP protocol for EIF3J antibody 10439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Death Dis

Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.

Authors - Anaïs Aulas
humanWB,IF,IP

J Cell Biol

eIF4GI links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy.

Authors - Ramírez-Valle Francisco F
  • KD Validated