|Positive WB detected in||mouse brain tissue, A2780 cells, mouse testis tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17069-1-AP targets EIF5A1/EIF5A2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EIF5A1/EIF5A2 fusion protein Ag10895|
|Full Name||eukaryotic translation initiation factor 5A2|
|Calculated molecular weight||153 aa, 17 kDa|
|Observed molecular weight||17 kDa|
|GenBank accession number||BC036072|
|Gene ID (NCBI)||56648|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Eukaryotic initiation factor 5A (EIF5A) plays an essential role in the viability of eukaryotic cells. EIF5A is known to act as a translation initiation factor specific for a small number of mRNAs, a cellular target of HIV-1 REV protein, and an exportin-4-dependent nuclear export cargo. It is also involved in mRNA turnover and the establishment of actin polarity. [PMID:16157662]. EIF5A2, one isoform of EIF5A, has a key at the level of mRNA turnoverby acting downstream of decapping. It also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity [PMID:14622290]. EIF5A2 shares 84% identity of amino acid sequence with EIF5A1 isoform, so EIF5A2 poly-antibody could recognize both EIF5A2 and EIF5A1.
EIF5A2 is a novel chemoresistance gene in breast cancer.
J Zhejiang Univ Sci B
Down-regulation of eIF5A-2 prevents epithelial-mesenchymal transition in non-small-cell lung cancer cells.
Knockdown of EIF5A2 inhibits the malignant potential of non-small cell lung cancer cells.
BMC Pulm Med
Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.
Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission.