EIF5A1/EIF5A2 Polyclonal antibody

• Featured Product
• KD/KO Validated

EIF5A1/EIF5A2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 17069-1-AP

Synonyms

eIF 5A 2, EIF 5A2, EIF5A1/EIF5A2, EIF5A2, eIF5AII

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Validation Data Gallery

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  WB analysis of mouse brain using 17069-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17069-1-AP (EIF5A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17069-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17069-1-AP (EIF5A2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 17069-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 17069-1-AP (EIF5A2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17069-1-AP

  IP result of anti-EIF5A1/EIF5A2 (IP:17069-1-AP, 3ug; Detection:17069-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human prostate cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 17069-1-AP (EIF5A2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 17069-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 17069-1-AP (EIF5A2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A2780 cells, mouse testis tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue, human colon cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

17069-1-AP targets EIF5A1/EIF5A2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF5A1/EIF5A2 fusion protein Ag10895
• Full Name: eukaryotic translation initiation factor 5A2
• Calculated Molecular Weight: 153 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC036072
• Gene Symbol: EIF5A2
• Gene ID (NCBI): 56648
• RRID: AB_2262009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic initiation factor 5A (EIF5A) plays an essential role in the viability of eukaryotic cells. EIF5A is known to act as a translation initiation factor specific for a small number of mRNAs, a cellular target of HIV-1 REV protein, and an exportin-4-dependent nuclear export cargo. It is also involved in mRNA turnover and the establishment of actin polarity. [PMID:16157662]. EIF5A2, one isoform of EIF5A, has a key at the level of mRNA turnoverby acting downstream of decapping. It also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity [PMID:14622290]. EIF5A2 shares 84% identity of amino acid sequence with EIF5A1 isoform, so EIF5A2 poly-antibody could recognize both EIF5A2 and EIF5A1.

Publications

Species	Application	Title
mouse	WB	PLoS Biol; Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission.; Authors - Dong Ma
mouse	WB	FASEB J; Global analyses of mRNA expression in human sensory neurons reveal eIF5A as a conserved target for inflammatory pain.; Authors - Rebecca Chase
human	WB	J Mol Biol; Eukaryotic Initiation Factor 5A2 Regulates Expression of Antiviral Genes.; Authors - Dorian Farache
human	WB	PLoS One; Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.; Authors - Jin Yan Y
human	WB	Breast Cancer; EIF5A2 is a novel chemoresistance gene in breast cancer.; Authors - Yu Liu ; KD Validated
human	WB	BMC Pulm Med; PMID: 25380840; Authors - Guodong Xu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah-Eve (Verified Customer) (02-19-2023)

Very Nice and specific signal, works well

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for wb and 1:200 for IF
• Cell Tissue Type: Human Pulmonary artery smooth muscle cells