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  • KD/KO Validated

EIF5A1/EIF5A2 Polyclonal antibody

EIF5A1/EIF5A2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17069-1-AP

Synonyms

eIF 5A 2, EIF 5A2, EIF5A1/EIF5A2, EIF5A2, eIF5AII



Tested Applications

Positive WB detected inmouse brain tissue, A2780 cells, mouse testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17069-1-AP targets EIF5A1/EIF5A2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF5A1/EIF5A2 fusion protein Ag10895
Full Name eukaryotic translation initiation factor 5A2
Calculated molecular weight 153 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC036072
Gene symbol EIF5A2
Gene ID (NCBI) 56648
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic initiation factor 5A (EIF5A) plays an essential role in the viability of eukaryotic cells. EIF5A is known to act as a translation initiation factor specific for a small number of mRNAs, a cellular target of HIV-1 REV protein, and an exportin-4-dependent nuclear export cargo. It is also involved in mRNA turnover and the establishment of actin polarity. [PMID:16157662]. EIF5A2, one isoform of EIF5A, has a key at the level of mRNA turnoverby acting downstream of decapping. It also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity [PMID:14622290]. EIF5A2 shares 84% identity of amino acid sequence with EIF5A1 isoform, so EIF5A2 poly-antibody could recognize both EIF5A2 and EIF5A1.

Protocols

Product Specific Protocols
WB protocol for EIF5A1/EIF5A2 antibody 17069-1-APDownload protocol
IHC protocol for EIF5A1/EIF5A2 antibody 17069-1-APDownload protocol
IP protocol for EIF5A1/EIF5A2 antibody 17069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Breast Cancer

EIF5A2 is a novel chemoresistance gene in breast cancer.

Authors - Yu Liu
  • KD Validated
humanIF

J Zhejiang Univ Sci B

Down-regulation of eIF5A-2 prevents epithelial-mesenchymal transition in non-small-cell lung cancer cells.

Authors - Xu Guo-Dong GD
humanWB,IHC

Oncol Lett

Knockdown of EIF5A2 inhibits the malignant potential of non-small cell lung cancer cells.

Authors - Cheng Chen
  • KD Validated
humanWB

BMC Pulm Med

PMID: 25380840

Authors - Guodong Xu
  • KD Validated
humanWB

PLoS One

Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.

Authors - Jin Yan Y
mouseWB

PLoS Biol

Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission.

Authors - Dong Ma