Tested Applications
Positive WB detected in | rat small intestine tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
28303-1-AP targets ENPP3/CD203c in WB, ELISA applications and shows reactivity with human, rat samples.
Tested Reactivity | human, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ENPP3/CD203c fusion protein Ag28478 Predict reactive species |
Full Name | ectonucleotide pyrophosphatase/phosphodiesterase 3 |
Calculated Molecular Weight | 875 aa, 100 kDa |
Observed Molecular Weight | 120-150 kDa |
GenBank Accession Number | BC146579 |
Gene Symbol | ENPP3 |
Gene ID (NCBI) | 5169 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O14638 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ENPP3/CD203c is part of ectonucleotide pyrophosphatases/phosphodiesterase (ENPP) family and plays a role in various physiological processes. Increased levels of ENPP3 have been observed in conditions, such as clear cell renal cell carcinoma, bile duct carcinoma, and colorectal cancer, positioning ENPP3 as a potential tumor marker (PMID: 38749434). Mice harboring a point mutant of ENPP3 that specifically abolishes its cGAMP hydrolase activity are more resistant to both primary tumor growth and metastasis in certain cancers (PMID: 38149831). High molecular weight band is the glycosylated form of ENPP3 (PMID: 27665743, 30271993)
Protocols
Product Specific Protocols | |
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WB protocol for ENPP3/CD203c antibody 28303-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |