ENPP3/CD203c Polyclonal antibody

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  WB analysis using 28303-1-AP

  rat small intestine tissue were subjected to SDS PAGE followed by western blot with 28303-1-AP (ENPP3/CD203c antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ENPP3/CD203c Polyclonal Antibody for WB, ELISA

Cat No. 28303-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

ENPP3, B10, CD203c, E NPP 3, PD IBETA

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Tested Applications

• Positive WB detected in: rat small intestine tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28303-1-AP targets ENPP3/CD203c in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ENPP3/CD203c fusion protein Ag28478
• Full Name: ectonucleotide pyrophosphatase/phosphodiesterase 3
• Calculated Molecular Weight: 875 aa, 100 kDa
• Observed Molecular Weight: 120-150 kDa
• GenBank Accession Number: BC146579
• Gene Symbol: ENPP3
• Gene ID (NCBI): 5169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14638
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENPP3/CD203c is part of ectonucleotide pyrophosphatases/phosphodiesterase (ENPP) family and plays a role in various physiological processes. Increased levels of ENPP3 have been observed in conditions, such as clear cell renal cell carcinoma, bile duct carcinoma, and colorectal cancer, positioning ENPP3 as a potential tumor marker (PMID: 38749434). Mice harboring a point mutant of ENPP3 that specifically abolishes its cGAMP hydrolase activity are more resistant to both primary tumor growth and metastasis in certain cancers (PMID: 38149831). High molecular weight band is the glycosylated form of ENPP3 (PMID: 27665743, 30271993)