EPHB1 Polyclonal antibody

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  WB analysis using 21762-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21762-1-AP (EPHB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

EPHB1 Polyclonal Antibody for WB, ELISA

Cat No. 21762-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:2.7.10.1, EK6, ELK, EPH like kinase 6, EPH receptor B1

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, SK-N-SH cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21762-1-AP targets EPHB1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPHB1 fusion protein Ag16347
• Full Name: EPH receptor B1
• Calculated Molecular Weight: 984 aa, 110 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC111744
• Gene Symbol: EPHB1
• Gene ID (NCBI): 2047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P54762
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPHB1, also named as EPHT2, NET, HEK6 and ELK, belongs to the protein kinase superfamily, Tyr protein kinase family, and Ephrin receptor subfamily. It is a receptor for members of the ephrin-B family. EPHB1 binds to ephrin-B1, -B2 and -B3. EPHB1 may be involved in cell-cell interactions in the nervous system. EPHB1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.