EPHX1 Monoclonal antibody, PBS Only

• 

  WB analysis using 66982-1-Ig (same clone as 66982-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66982-1-Ig (same clone as 66982-1-PBS)

  WB result of EPHX1 antibody (66982-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX1 transfected HeLa cells. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 66982-1-Ig (same clone as 66982-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 66982-1-Ig (same clone as 66982-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66982-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

EPHX1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66982-1-PBS

Clone No.1B6A2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Synonyms

EPHX, EPHX1, EPOX, Epoxide hydratase, Epoxide hydrolase 1, MEH, Microsomal epoxide hydrolase

---

Product Information

66982-1-PBS targets EPHX1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25820

  Product name: Recombinant human EPHX1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 257-455 aa of BC003567

  Sequence: FSTLTLLLGQRFGRFLGLTERDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSTWTNTEFRYLEDGGLERKFSLDDLLTNVMLYWTTGTIISSQRFYKENLGQGWMTQKHERMKVYVPTGFSAFPFELLHTPEKWVRFKYPKLISYSYMVRGGHFAAFEEPELLAQDIRKFLSVLERQ

• Full Name: epoxide hydrolase 1, microsomal (xenobiotic)
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC003567
• Gene Symbol: EPHX1
• Gene ID (NCBI): 2052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07099
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EPHX1, also known as EPHX and EPOX, belongs to the peptidase S33 family. EPHX1 is an evolutionarily highly conserved biotransformation enzyme expressed in nearly all tissues and localized mainly in the microsomal fraction of the endoplasmic reticulum of eukaryotic cells. EPHX1 plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. The calculated molecular weight of EPHX1 is 53 kDa (PMID: 26216302).