Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HEK-293T cells, mouse colon tissue, rat cerebellum tissue |
| Positive IP detected in | mouse large intestine tissue, HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, mouse brain tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10833-1-AP targets EPHX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1283 Product name: Recombinant human EPHX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 212-555 aa of BC013874 Sequence: ELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAELEQNLSRTFKSLFRASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLDSDARNPPVVSKM Predict reactive species |
| Full Name | epoxide hydrolase 2, cytoplasmic |
| Calculated Molecular Weight | 63 kDa |
| Observed Molecular Weight | 63 kDa |
| GenBank Accession Number | BC013874 |
| Gene Symbol | EPHX2 |
| Gene ID (NCBI) | 2053 |
| RRID | AB_2098246 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P34913 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EPHX2 antibody 10833-1-AP | Download protocol |
| IHC protocol for EPHX2 antibody 10833-1-AP | Download protocol |
| IP protocol for EPHX2 antibody 10833-1-AP | Download protocol |
| WB protocol for EPHX2 antibody 10833-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EPHX2 (soluble epoxide hydrolase 2) antibody (Cat# 10833-1-AP) has accumulated 31 citations in journals including Nature, Advanced Science, ACS Central Science, Journal of Medicinal Chemistry, Free Radical Biology and Medicine, iScience, and Geroscience. Associated research fields encompass inflammation, renal injury, cardiovascular disease, cancer (hepatocellular, colon, renal cell, glioblastoma), ferroptosis, lipid/arachidonic acid metabolism, neurology, skin aging, and tissue repair, with applications in WB, IHC, and IF across mouse, rat, human, and chicken models.
| Species | Application | Title |
|---|---|---|
Exploration (Beijing) Time-of-Day Defines the Risk of Thermally Abused Frying Oil to Renal Injury by Modulating the Diurnal Dynamics of Oxylipins. | ||
Adv Sci (Weinh) CYP4X1/sEH-Dependent Endocannabinoid Metabolism Drives Fibroblast-Mediated Immunosuppression to Limit Immunotherapy in Colon Cancer | ||
NPJ Aging Decoding skin aging: the role of KNG1 in collagen and elastic fibre degradation. | ||
Int J Biol Sci Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury | ||
Phytomedicine Qingfei Tongluo Formula attenuates COPD-associated ferroptosis by restoring arachidonic acid metabolic balance via dual inhibition of prostaglandin-endoperoxide synthase 2 and soluble epoxide hydrolase 2. |
Reviews
What customers say
The single review for this antibody is highly positive, rated 5 stars. The reviewer reports consistent, reliable performance in Western blot applications, stating it "works every time." The antibody was used at a 1:1000 dilution on human iPSC-derived neurons, with a representative blot image provided to support the result. Overall, the feedback highlights dependable and reproducible Western blot performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Dhruv (Verified Customer) (10-30-2025) | This antibody works everytime for my western blot
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