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EPHX2 Polyclonal antibody

EPHX2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10833-1-AP

Synonyms

CEH, Cytosolic epoxide hydrolase, EPHX2, Epoxide hydratase, Epoxide hydrolase 2, SEH, Soluble epoxide hydrolase



Tested Applications

Positive WB detected inHEK-293 cells, mouse colon tissue, rat cerebellum tissue, HEK-293T cells, A549 cells
Positive IP detected inmouse large intestine tissue, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10833-1-AP targets EPHX2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EPHX2 fusion protein Ag1283
Full Name epoxide hydrolase 2, cytoplasmic
Calculated molecular weight 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC013874
Gene symbol EPHX2
Gene ID (NCBI) 2053
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. A number of single nucleotide polymorphisms (SNPs) in human EPHX2 have been linked to cardiovascular disease risk, including increased risk of coronary heart disease, hyperlipoproteinemia, and type-2 diabetes (PMID: 14732757, 16595607, 14673705, 15845398, 17460077). It was observed in many tissues with the band of 63 kDa in the western blot. It has also been reported that the N-terminal domain might promote dimerization of EPHX2 (PMID:21553642).

Protocols

Product Specific Protocols
WB protocol for EPHX2 antibody 10833-1-APDownload protocol
IHC protocol for EPHX2 antibody 10833-1-APDownload protocol
IF protocol for EPHX2 antibody 10833-1-APDownload protocol
IP protocol for EPHX2 antibody 10833-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenWB

Arch Biochem Biophys

Mitochondrial P450-dependent arachidonic acid metabolism by TCDD-induced hepatic CYP1A5; conversion of EETs to DHETs by mitochondrial soluble epoxide hydrolase.

Authors - Labitzke Erin M EM