ERBB4 Polyclonal antibody

ERBB4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 19943-1-AP


4ICD, E4ICD, ERBB4, HER4, p180erbB4, s80HER4

Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells, rat brain tissue
Positive IHC detected inmouse lung tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19943-1-AP targets ERBB4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
Calculated Molecular Weight 147 kDa
Observed Molecular Weight 180 kDa, 147 kDa
GenBank Accession NumberNM_001042599
Gene Symbol ERBB4
Gene ID (NCBI) 2066
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERBB4, also named p180erbB4 and HER4, belongs to the protein kinase superfamily, Tyr protein kinase family, and EGF receptor subfamily. ERBB4 specifically binds and it is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin, and NTAK. Interaction with these factors induces cell differentiation. ERBB4 is not activated by EGF, TGF-A, and amphiregulin. ERBB4 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody recognizes the C-term of ERBB4. Alternatively spliced variants that encode different protein isoforms with a calculated molecular weight of 144-147 kDa have been described. The apparent molecular weight of full-length ERBB4 is 150-200 kDa due to glycosylation and phosphorylation. Full-length ERBB4 can be cleaved into an 80-kDa ERBB4 intracellular fragment and a 120-kDa extracellular fragment (PMID: 11343971; 16251361).


Product Specific Protocols
WB protocol for ERBB4 antibody 19943-1-APDownload protocol
IHC protocol for ERBB4 antibody 19943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Sci (Weinh)

Neuregulin4 Acts on Hypothalamic ErBb4 to Excite Oxytocin Neurons and Preserve Metabolic Homeostasis

Authors - Yi Zhang

Respir Res

Identification of targetable kinases in idiopathic pulmonary fibrosis.

Authors - Hisao Higo

Front Oncol

DNAJC12 as a Mediator Between ESR1 and ERBB4 in Breast Carcinoma Cells.

Authors - Mianjie Lin

J Neuroinflammation

EGFR transactivation contributes to neuroinflammation in Streptococcus suis meningitis.

Authors - Xiao-Pei Yang

Cell Mol Neurobiol

Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature Oligodendrocytes

Authors - Dou Ye

DNA Cell Biol

AK003290 Protects Myocardial Cells Against Apoptosis and Promotes Cardiac Function Recovery Via miR-539-3p/ErbB4 Axis in Ischemic-Reperfusion Injury.

Authors - Yong Tang