|Positive WB detected in||mouse brain tissue, MCF-7 cells, rat brain tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
19943-1-AP targets ERBB4 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)|
|Calculated molecular weight||147 kDa|
|Observed molecular weight||180 kDa, 147 kDa|
|GenBank accession number||NM_001042599|
|Gene ID (NCBI)||2066|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ERBB4, also named as p180erbB4 and HER4, belongs to the protein kinase superfamily, Tyr protein kinase family and EGF receptor subfamily. ERBB4 specifically binds and it is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. ERBB4 is not activated by EGF, TGF-A, and amphiregulin. ERBB4 catalyze the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody recognize the C-term of ERBB4. Alternatively spliced variants which encode different protein isoforms with calculated molecular weight of 144-147 kDa have been described. The apparent molecular weight of full-length ERBB4 is 150-200 kDa due to glycosylation and phosphorylation. Full-length ERBB4 can be cleaved into an 80-kDa ERBB4 intracellular fragment and a 120-kDa extracellular fragment (PMID: 11343971; 16251361).
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MicroRNA-936/ERBB4/Akt axis exhibits anticancer properties of gastric cancer through inhibition of cell proliferation, migration, and invasion.
EGFR transactivation contributes to neuroinflammation in Streptococcus suis meningitis.
DNAJC12 as a Mediator Between ESR1 and ERBB4 in Breast Carcinoma Cells.