|Positive WB detected in||HSC-T6 cells, Jurkat cells, ROS1728 cells, RAW 264.7 cells, HeLa cells|
|Positive IHC detected in||human liver cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
67170-1-Ig targets ERK1/2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Tested Reactivity||Human, Mouse, Rat|
|Cited Reactivity||human, mouse, rabbit, rat|
|Host / Isotype||Mouse / IgG2a|
|Full Name||mitogen-activated protein kinase 3|
|Calculated molecular weight||38-43 kDa|
|Observed molecular weight||38-44 kDa|
|GenBank accession number||NM_002746|
|Gene ID (NCBI)||5595|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
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S (Verified Customer) (05-18-2020)