ERK1/2 Monoclonal antibody
ERK1/2 Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat and More (4)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1A3D3
Cat no : 67170-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HSC-T6 cells, Jurkat cells, ROS1728 cells, RAW 264.7 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 21 publications below |
| IHC | See 1 publications below |
Product Information
67170-1-Ig targets ERK1/2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse, rabbit, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | mitogen-activated protein kinase 3 |
| Calculated molecular weight | 38-43 kDa |
| Observed molecular weight | 38-44 kDa |
| GenBank accession number | NM_002746 |
| Gene symbol | MAPK3 |
| Gene ID (NCBI) | 5595 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERK1/2 antibody 67170-1-Ig | Download protocol |
| IHC protocol for ERK1/2 antibody 67170-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bone AKAP2 overexpression modulates growth plate chondrocyte functions through ERK1/2 signaling. | ||
Cell Death Discov A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein. | ||
J Renin Angiotensin Aldosterone Syst PM2.5 inducing myocardial fibrosis mediated by Ang II/ERK1/2/TGF-β1 signaling pathway in mice model. | ||
Eur Rev Med Pharmacol Sci Blocking VRK2 suppresses pulmonary adenocarcinoma progression via ERK1/2/AKT signal pathway by targeting miR-145-5p. | ||
Onco Targets Ther RPL38 Regulates the Proliferation and Apoptosis of Gastric Cancer via miR-374b-5p/VEGF Signal Pathway. | ||
J Tissue Eng Regen Med The function and mechanism of the miR-210-3p/KRAS axis in bone marrow-derived mesenchymal stem cell from patients with osteoporosis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH S (Verified Customer) (05-18-2020) | Excellent Antibody
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