ERK1/2 Monoclonal antibody

ERK1/2 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (4)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

33

CloneNo.

1A3D3

Reviews (1)

Cat no : 67170-1-Ig

Synonyms

ERK, ERK 1, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

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Validation Data Gallery

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  WB analysis of ROS1728 using 67170-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67170-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 67170-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67170-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 67170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67170-1-Ig (ERK1/2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67170-1-Ig (ERK1/2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 32 publications below
• IHC: See 1 publications below

Product Information

67170-1-Ig targets ERK1/2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rabbit, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase 3
• Calculated molecular weight: 38-43 kDa
• GenBank accession number: NM_002746
• Gene symbol: MAPK3
• Gene ID (NCBI): 5595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publications

Species	Application	Title
human	WB	Bone; AKAP2 overexpression modulates growth plate chondrocyte functions through ERK1/2 signaling.; Authors - Bing Wang
human	WB	Cell Death Discov; A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein.; Authors - Pengyun Li
rat	WB	J Renin Angiotensin Aldosterone Syst; PM2.5 inducing myocardial fibrosis mediated by Ang II/ERK1/2/TGF-β1 signaling pathway in mice model.; Authors - Xiwen Zang
human	WB	Eur Rev Med Pharmacol Sci; Blocking VRK2 suppresses pulmonary adenocarcinoma progression via ERK1/2/AKT signal pathway by targeting miR-145-5p.; Authors - Y Mu
mouse	WB	Phytochemistry; Diterpenoid alkaloids from Delphinium forrestii var. viride and their anti-inflammation activity.; Authors - Zhuorui Song
human	WB	Onco Targets Ther; RPL38 Regulates the Proliferation and Apoptosis of Gastric Cancer via miR-374b-5p/VEGF Signal Pathway.; Authors - Hanshu Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (05-18-2020)

Excellent Antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: AC16

ERK1/2 Antibody Western Blot validation (1:1000 dilution) in AC16 (Cat no:67170-1-Ig)