ERK1/2 Polyclonal antibody

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• ERK1/2 Polyclonal antibody (11257-1-AP)

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• KD/KO Validated

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11257-1-AP

Publications(836)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

ERK, ERK1, ERK2, EC:2.7.11.24, ERK-1

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  WB analysis using 11257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 11257-1-AP

  WB result of ERK1/2 antibody (11257-1-AP, 1:1000) with HeLa and HEK293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 11257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11257-1-AP

  WB result of ERK1/2 antibody (11257-1-AP, 1:2000) with si-Control and si-ERK1/2 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 11257-1-AP

  IP result of anti-ERK1/2 (IP:11257-1-AP, 4ug; Detection:11257-1-AP 1:3000) with HEK-293 cells lysate 1440 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 11257-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 11257-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ERK1/2 antibody (11257-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 11257-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ERK1/2 (11257-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PC-12 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse lung tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11257-1-AP targets ERK1/2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, chicken, bovine, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1759

  Product name: Recombinant human ERK1/2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-379 aa of BC013992

  Sequence: MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP

• Full Name: mitogen-activated protein kinase 3
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 38-44 kDa
• GenBank Accession Number: BC013992
• Gene Symbol: ERK1
• Gene ID (NCBI): 5595
• RRID: AB_2139822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P27361
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Signal Transduct Target Ther; A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics; Authors - Zhiheng Rao
Human	WB	Signal Transduct Target Ther; Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells; Authors - Pengju Wang
human	WB	Mol Cancer; CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer; Authors - Fei Xing
human	WB	Mol Cancer; A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer; Authors - Runjie Song
human	WB	Gastroenterology; M6A-mediated upregulation of FZD10 regulates liver cancer stem cells properties and lenvatinib resistance through WNT/β-catenin and Hippo signaling pathways; Authors - Jinghan Wang
mouse	WB,IP,IHC,IF,FC (Intra),ELISA,CoIP	Adv Mater; Ultrasound-Activated Sonophage Synergizes Sonodynamic Therapy and Saltoptosis for Solid Tumor Eradication.; Authors - Kerong Chen

Reviews

5.0 out of 5

3 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Han (Verified Customer) (08-11-2026)

This product is performing very well, and the pricing is exactly where it needs to be.

• Applications: Other

Charlotte (Verified Customer) (08-15-2024)

The company suggests us this ERK ab as the other one we bought from them was not working. I overlaid ERK with pERK and it matches nicely. Satisfied of this antibody. Done in milk.

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: HT29

ERK1/2 Antibody Western Blot validation (1/5000 dilution) in HT29 (Cat no:11257-1-AP)

XB (Verified Customer) (10-29-2020)

A reliable ab to detect total ERK1/2 by Western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain

ERK1/2 Antibody Western Blot validation (1:3000 dilution) in mouse brain (Cat no:11257-1-AP)