Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody

Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

139

Reviews (1)

Cat no : 28733-1-AP

Synonyms

ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, Phospho-ERK1/2 (Thr202/Tyr204), PRKM3

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Validation Data Gallery

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  WB analysis using 28733-1-AP

  Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (ERK1/2-phospho-Thr202/Tyr204) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 28733-1-AP

  Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:4500 incubated at 4℃ overnight.

• 

  IP experiment of PC-3 using 28733-1-AP

  IP result of anti-Phospho-ERK1/2 (Thr202/Tyr204)(IP:28733-1-AP, 2ug; Detection:28733-1-AP 1:1000) with Calyculin A treated PC-3 cells lysate 1552 ug.

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Tested Applications

• Positive WB detected in: Calyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells
• Positive IP detected in: Calyculin A treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:9000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 135 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase 3
• Calculated molecular weight: 38-43 kDa
• Observed molecular weight: 38-43 kDa
• GenBank accession number: NM_002746
• Gene symbol: MAPK3
• Gene ID (NCBI): 5595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. The antibody recognizes ERK2 phosphorylation sites Thr185 and Tyr187.

Publications

Species	Application	Title
human	WB	Mol Cancer; Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression.; Authors - Zhenzhen Pan
human	WB	Proc Natl Acad Sci U S A; Progesterone activates GPR126 to promote breast cancer development via the Gi pathway.; Authors - Wentao An
mouse	WB	J Hazard Mater; Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders.; Authors - Tianxin Zhao
human	WB	Front Immunol; Dysfunction of CCR1+ decidual macrophages is a potential risk factor in the occurrence of unexplained recurrent pregnancy loss; Authors - Yifei Sang
human	WB	Oncogene; The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis; Authors - Yapeng Wang
mouse	WB	J Transl Med; Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways; Authors - Xin-Sen Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

nice band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000