Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody

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  WB analysis using 28733-1-AP

  Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (ERK1/2-phospho-Thr202/Tyr204) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 28733-1-AP

  Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:4500 incubated at 4℃ overnight.

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  WB analysis using 28733-1-AP

  Non-treated and PMA treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 28733-1-AP

  IP result of anti-Phospho-ERK1/2 (Thr202/Tyr204) (IP:28733-1-AP, 2ug; Detection:28733-1-AP 1:1000) with Calyculin A treated PC-3 cells lysate 1552 ug.

• Featured Product
• KD/KO Validated

Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal Antibody for WB, IP, ELISA

Cat No. 28733-1-AP

Publications(471)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ERK1/2 (Thr202/Tyr204), p ERK, p ERK1/2, p ERK1/2 (Thr202/Tyr204), p-ERK

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, Calyculin A treated HEK-293T cells
• Positive IP detected in: Calyculin A treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 457 publications below
• IHC: See 29 publications below
• IF: See 15 publications below

Product Information

28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase 3
• Calculated Molecular Weight: 38-43 kDa
• Observed Molecular Weight: 38-43 kDa
• GenBank Accession Number: NM_002746
• Gene Symbol: ERK1
• Gene ID (NCBI): 5595
• RRID: AB_2881202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P27361
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. The antibody recognizes ERK2 phosphorylation sites Thr185 and Tyr187.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis; Authors - Chuan Yang
human	WB	Mol Cancer; Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression.; Authors - Zhenzhen Pan
mouse	WB	Biomaterials; Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure; Authors - Lingjun Tong
human	WB	Exp Mol Med; Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer; Authors - Yanmei Peng
human	WB	Redox Biol; Twin defect-rich Pt ultrathin nanowire nanozymes alleviate inflammatory skin diseases by scavenging reactive oxygen species; Authors - He Zhao
mouse	WB	EMBO Mol Med; Luteolin detoxifies DEHP and prevents liver injury by degrading Uroc1 protein in mice; Authors - Huiting Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-15-2024)

The last (most intense) band correspond to pERK. It worked nicely

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: HT29

Phospho-ERK1/2 (Thr202/Tyr204) Antibody Western Blot validation (1/2000 dilution) in HT29 (Cat no:28733-1-AP)

FH

Alexandra (Verified Customer) (06-06-2023)

P19 cells were aggregated in bacteria petri dishes for 6h (point1) and 12 and 24 h (point 2 and 3) in the presence of 1uM RA (retinoic acid). For neuronal differentiation the RA treatment lasts for 4 days, the cells are trypsinized and cultured in serum free neurobasal medium supplemented with N2, glutamax and penstrep. Cell aggregates were collected by centrifugation, washed in 1X PBS and lysed in denaturing lysis buffer. For WB i used PVDF, blocking in 5%milk and the antibody incubation was O/N at 4C. Please do not include the Parp1 KO annotation if you will use this data. In the PDF you can find the ladder and the exposition as well. If the data did not get uploaded (i cannot tell) let me know and i will send it by email.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: P19 derived embryonic bodies

FH

Macarena Lucia (Verified Customer) (10-17-2022)

nice band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000