Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody

Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat and More (4)





Cat no : 28733-1-AP


ERK 1, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, Phospho-ERK1/2 (Thr202/Tyr204), PRKM3

Tested Applications

Positive WB detected inNIH/3T3 cells, Calyculin A treated HEK-293T cells
Positive IP detected inCalyculin A treated PC-3 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, sheep, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase 3
Calculated Molecular Weight 38-43 kDa
Observed Molecular Weight 38-43 kDa
GenBank Accession NumberNM_002746
Gene Symbol MAPK3
Gene ID (NCBI) 5595
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. The antibody recognizes ERK2 phosphorylation sites Thr185 and Tyr187.


Product Specific Protocols
WB protocol for Phospho-ERK1/2 (Thr202/Tyr204) antibody 28733-1-APDownload protocol
IP protocol for Phospho-ERK1/2 (Thr202/Tyr204) antibody 28733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Alexandra (Verified Customer) (06-06-2023)

P19 cells were aggregated in bacteria petri dishes for 6h (point1) and 12 and 24 h (point 2 and 3) in the presence of 1uM RA (retinoic acid). For neuronal differentiation the RA treatment lasts for 4 days, the cells are trypsinized and cultured in serum free neurobasal medium supplemented with N2, glutamax and penstrep. Cell aggregates were collected by centrifugation, washed in 1X PBS and lysed in denaturing lysis buffer. For WB i used PVDF, blocking in 5%milk and the antibody incubation was O/N at 4C. Please do not include the Parp1 KO annotation if you will use this data. In the PDF you can find the ladder and the exposition as well. If the data did not get uploaded (i cannot tell) let me know and i will send it by email.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: P19 derived embryonic bodies

Macarena Lucia (Verified Customer) (10-17-2022)

nice band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000