Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody

Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 28733-1-AP

Synonyms

ERK 1, ERK1, ERK1/2, ERK1/2 T202, ERK1/2 T202/Y204), ERK1/2 Thr202, ERK1/2 Thr202/Tyr204, ERK1/2 Tyr204, ERK1/2 Y204, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, Phospho-ERK1/2 (Thr202/Tyr204), PRKM3



Tested Applications

Positive WB detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase 3
Calculated molecular weight 38-43 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberNM_002746
Gene symbol MAPK3
Gene ID (NCBI) 5595
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1.

Protocols

Product Specific Protocols
WB protocol for Phospho-ERK1/2 (Thr202/Tyr204) antibody 28733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

IL-21 mediates microRNA-423-5p /claudin-5 signal pathway and intestinal barrier function in inflammatory bowel disease.

Authors - Mu Wang
mouseWB

Biomed Res Int

Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects.

Authors - Zuo-Wei Pei
humanWB

Arch Physiol Biochem

CPSF1 mediates retinal vascular dysfunction in diabetes mellitus via the MAPK/ERK pathway.

Authors - Jingyi Zhang
mouseWB

Reprod Toxicol

Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult mice.

Authors - Shuxian Wang
humanWB

Toxicol Appl Pharmacol

Berberine-photodynamic therapy sensitizes melanoma cells to cisplatin-induced apoptosis through ROS-mediated P38 MAPK pathways.

Authors - Xiaotong Wang
humanWB

J Biochem Mol Toxicol

Vicenin-2 inhibits the Helicobacterium pylori infection associated gastric carcinogenic events through modulation of PI3K/AKT and Nrf2 signaling in GES-1 cells.

Authors - Yifeng Zhang