Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody
Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
139
Cat no : 28733-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Calyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells |
Positive IP detected in | Calyculin A treated PC-3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:9000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 135 publications below |
IHC | See 3 publications below |
IF | See 3 publications below |
Product Information
28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | mitogen-activated protein kinase 3 |
Calculated molecular weight | 38-43 kDa |
Observed molecular weight | 38-43 kDa |
GenBank accession number | NM_002746 |
Gene symbol | MAPK3 |
Gene ID (NCBI) | 5595 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. The antibody recognizes ERK2 phosphorylation sites Thr185 and Tyr187.
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-ERK1/2 (Thr202/Tyr204) antibody 28733-1-AP | Download protocol |
IP protocol for Phospho-ERK1/2 (Thr202/Tyr204) antibody 28733-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cancer Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression. | ||
Proc Natl Acad Sci U S A Progesterone activates GPR126 to promote breast cancer development via the Gi pathway. | ||
J Hazard Mater Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders. | ||
Front Immunol Dysfunction of CCR1+ decidual macrophages is a potential risk factor in the occurrence of unexplained recurrent pregnancy loss | ||
Oncogene The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axis | ||
J Transl Med Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Macarena Lucia (Verified Customer) (10-17-2022) | nice band
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