Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody
Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Human, mouse, rat and More (2)
WB, IP, IHC, IF, ELISA
Cat no : 28733-1-AP
|Positive WB detected in||Calyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells|
|Positive IP detected in||Calyculin A treated PC-3 cells|
|Western Blot (WB)||WB : 1:1000-1:9000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Sample-dependent, check data in validation data gallery|
|WB||See 135 publications below|
|IHC||See 3 publications below|
|IF||See 3 publications below|
28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||mitogen-activated protein kinase 3|
|Calculated molecular weight||38-43 kDa|
|Observed molecular weight||38-43 kDa|
|GenBank accession number||NM_002746|
|Gene ID (NCBI)||5595|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. The antibody recognizes ERK2 phosphorylation sites Thr185 and Tyr187.
|Product Specific Protocols|
|WB protocol for Phospho-ERK1/2 (Thr202/Tyr204) antibody 28733-1-AP||Download protocol|
|IP protocol for Phospho-ERK1/2 (Thr202/Tyr204) antibody 28733-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression.
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J Transl Med
Angiotensin-(1-7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways
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Macarena Lucia (Verified Customer) (10-17-2022)