Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody
Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal Antibody for IP, WB, ELISA
Cat no : 28733-1-AP
|Positive WB detected in||Calyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells|
|Positive IP detected in||Calyculin A treated PC-3 cells|
|Western Blot (WB)||WB : 1:1000-1:9000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Sample-dependent, check data in validation data gallery|
28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||mitogen-activated protein kinase 3|
|Calculated molecular weight||38-43 kDa|
|Observed molecular weight||38-43 kDa|
|GenBank accession number||NM_002746|
|Gene ID (NCBI)||5595|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1.
Aging (Albany NY)
IL-21 mediates microRNA-423-5p /claudin-5 signal pathway and intestinal barrier function in inflammatory bowel disease.
Biomed Res Int
Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects.
Acta Biochim Biophys Sin (Shanghai)
Serum proteome profiling provides a deep understanding of the 'gut-liver axis' in relation to liver injury and regeneration.
Cell Death Dis
Autophagy-associated circular RNA hsa_circ_0007813 modulates human bladder cancer progression via hsa-miR-361-3p/IGF2R regulation.
Biomed Res Int
Oridonin Promotes Apoptosis and Restrains the Viability and Migration of Bladder Cancer by Impeding TRPM7 Expression via the ERK and AKT Signaling Pathways.
ACS Chem Neurosci
Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus