Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody

Phospho-ERK1/2 (Thr202/Tyr204) Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

30

Cat no : 28733-1-AP

Synonyms

ERK 1, ERK1, ERK1/2, ERK1/2 T202, ERK1/2 T202/Y204), ERK1/2 Thr202, ERK1/2 Thr202/Tyr204, ERK1/2 Tyr204, ERK1/2 Y204, ERT2, HS44KDAP, HUMKER1A, Insulin stimulated MAP2 kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, Phospho-ERK1/2 (Thr202/Tyr204), PRKM3

---

Validation Data Gallery

• 

  WB analysis using 28733-1-AP

  Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (Phospho-ERK1/2 (Thr202/Tyr204) antibody) at dilution of 1:4500 incubated at 4℃ overnight.

• 

  WB analysis using 28733-1-AP

  Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28733-1-AP (ERK1/2-phospho-Thr202/Tyr204) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  IP experiment using 28733-1-AP

  IP result of anti-Phospho-ERK1/2 (Thr202/Tyr204)(IP:28733-1-AP, 2ug; Detection:28733-1-AP 1:1000) with Calyculin A treated PC-3 cells lysate 1552 ug.

---

Tested Applications

• Positive WB detected in: Calyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells
• Positive IP detected in: Calyculin A treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:9000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 29 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

28733-1-AP targets Phospho-ERK1/2 (Thr202/Tyr204) in WB, IP, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase 3
• Calculated molecular weight: 38-43 kDa
• Observed molecular weight: 38-43 kDa
• GenBank accession number: NM_002746
• Gene symbol: MAPK3
• Gene ID (NCBI): 5595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); IL-21 mediates microRNA-423-5p /claudin-5 signal pathway and intestinal barrier function in inflammatory bowel disease.; Authors - Mu Wang
mouse	WB	Biomed Res Int; Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice via Its Anti-inflammatory and Antipyroptotic Effects.; Authors - Zuo-Wei Pei
mouse	IF	Acta Biochim Biophys Sin (Shanghai); Serum proteome profiling provides a deep understanding of the 'gut-liver axis' in relation to liver injury and regeneration.; Authors - Ling Leng
human	WB	Cell Death Dis; Autophagy-associated circular RNA hsa_circ_0007813 modulates human bladder cancer progression via hsa-miR-361-3p/IGF2R regulation.; Authors - Zheyu Zhang
human	WB	Biomed Res Int; Oridonin Promotes Apoptosis and Restrains the Viability and Migration of Bladder Cancer by Impeding TRPM7 Expression via the ERK and AKT Signaling Pathways.; Authors - Xianping Che
mouse	WB	ACS Chem Neurosci; Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus; Authors - Yan Sun