ESPN Polyclonal antibody, PBS Only

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  WB analysis using 20717-1-AP (same clone as 20717-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20717-1-AP (ESPN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20717-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 20717-1-AP (same clone as 20717-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20717-1-AP (ESPN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 20717-1-AP (same clone as 20717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 20717-1-AP (ESPN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20717-1-PBS in a different storage buffer formulation.

ESPN Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 20717-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

LP2654, Espin, DFNB36

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Product Information

20717-1-PBS targets ESPN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: espin
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 30-33 kDa, 110 kDa
• GenBank Accession Number: NM_031475
• Gene Symbol: ESPN
• Gene ID (NCBI): 83715
• RRID: AB_10858327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: B1AK53
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ESPN, also named as DFNB36, is a multifunctional actin-bundling protein. ESPN plays a major role in regulating the organization, dimensions, dynamics and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in variouS mechanosensory and chemosensory cells. Defects in ESPN are the cause of deafness autosomal recessive type 36 (DFNB36). Defects in ESPN are the cause of deafness autosomal dominant without vestibular involvement (DFNAWVI). The antibody is specific to ESPN.