|Positive WB detected in||NIH/3T3 cells, human brain tissue, SH-SY5Y cells, mouse brain tissue, mouse testis tissue, PC-3 cells, MCF-7 cells|
|Positive IHC detected in||human cervix tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14007-1-AP targets ESR2 in WB, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ESR2 fusion protein Ag5103|
|Full Name||estrogen receptor 2 (ER beta)|
|Calculated molecular weight||59 kDa|
|Observed molecular weight||55-60 kDa|
|GenBank accession number||BC024181|
|Gene ID (NCBI)||2100|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa(PMID:14766008).
Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.
Establishment and evaluation of a novel mouse model of peri/postmenopausal depression.
Mol Med Rep
Saikosaponin‑D improves fear memory deficits in ovariectomized rats via the action of estrogen receptor‑α in the hippocampus.
J Steroid Biochem Mol Biol
High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.
Biochem Cell Biol
Rutin attenuates cerebral ischemia/reperfusion injury in ovariectomized rats via estrogen receptor-mediated BDNF-TrkB and NGF-TrkA signaling.
J Biomed Sci
Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kenneth (Verified Customer) (01-08-2020)