ESR2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ESR2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

42

Reviews (1)

Cat no : 14007-1-AP

Synonyms

ER BETA, Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

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Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 14007-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14007-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14007-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14007-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14007-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 14007-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14007-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14007-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14007-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, human brain tissue, SH-SY5Y cells, mouse brain tissue, mouse testis tissue, PC-3 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 30 publications below
• IHC: See 16 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Product Information

14007-1-AP targets ESR2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ESR2 fusion protein Ag5103
• Full Name: estrogen receptor 2 (ER beta)
• Calculated molecular weight: 59 kDa
• Observed molecular weight: 55-60 kDa
• GenBank accession number: BC024181
• Gene symbol: ESR2
• Gene ID (NCBI): 2100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa(PMID:14766008).

Publications

Species	Application	Title
human	IF	Hum Reprod; Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.; Authors - Yanling Gou
mouse	WB	Heliyon; Establishment and evaluation of a novel mouse model of peri/postmenopausal depression.; Authors - Ling Zhang
	WB	Mol Med Rep; Saikosaponin‑D improves fear memory deficits in ovariectomized rats via the action of estrogen receptor‑α in the hippocampus.; Authors - Lina Liu
mouse, human	WB	J Steroid Biochem Mol Biol; High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.; Authors - Rui Wang
rat	WB	Biochem Cell Biol; Rutin attenuates cerebral ischemia/reperfusion injury in ovariectomized rats via estrogen receptor-mediated BDNF-TrkB and NGF-TrkA signaling.; Authors - Hong Liu
mouse	WB	J Biomed Sci; Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.; Authors - Chunfang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenneth (Verified Customer) (01-08-2020)

N/A

• Applications: Western Blot,

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