Name: ESR2 Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee

ESR2 Antibody IHC human cervix tissue 14007-1-AP

Immunohistochemical of paraffin-embedded human cervix using 14007-1-AP(ESR2 antibody) at dilution of 1:50 (under 10x lens)

ESR2 Antibody WB NIH/3T3 cells 14007-1-AP

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP( ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Species specificity:

human, mouse, rat

Positive WB detected in:

NIH/3T3 cells, human brain tissue, SH-SY5Y cells, mouse brain tissue, mouse testis tissue, PC-3 cells, MCF-7 cells

Positive IHC detected in:

human cervix tissue, human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution:

WB : 1:1000-1:4000

IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ESR2 fusion protein Ag5103

Full name:

estrogen receptor 2 (ER beta)

Calculated molecular weight:

59 kDa

Observed molecular weight:

55-60 kDa

GenBank accession number:

BC024181

Gene ID (NCBI):

2100

Gene symbol

ESR2

Synonyms

ER BETA, Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

Background

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa(PMID:14766008).

Showing 1- [6] of [15] Publications

Author:		Nan Zheng
Pubmed ID:		26767948
Journal:		Arch Biochem Biophys
Title:		ERβ up-regulation was involved in silibinin-induced growth inhibition of human breast cancer MCF-7 cells.
Application:		WB
KD validated
Species:		human

Author:		Jing Yang
Pubmed ID:		30729588
Journal:		Phytother Res
Title:		Silibinin protects rat pancreatic β-cell through up-regulation of estrogen receptors' signaling against amylin- or Aβ1-42 -induced reactive oxygen species/reactive nitrogen species generation.
Application:		WB
KD validated
Species:		rat

Author:		He Yu-qi YQ
Pubmed ID:		23143558
Journal:		Exp Mol Med
Title:		Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells.
Application:		WB

Species:		human

Author:		Tang Hexiao H
Pubmed ID:		23649836
Journal:		Int J Cancer
Title:		Estrogen and insulin-like growth factor synergistically promote the development of lung adenocarcinoma in mice.
Application:		IHC

Species:		mouse

Author:		Chunfang Zhang
Pubmed ID:		24742230
Journal:		J Biomed Sci
Title:		Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.
Application:		WB

Species:		mouse

Author:		Chao Fu
Pubmed ID:		25655087
Journal:		J Cell Physiol
Title:		Alpha-Lipoic Acid Promotes Osteoblastic Formation in H2 O2 -Treated MC3T3-E1 Cells and Prevents Bone Loss in Ovariectomized Rats.
Application:		WB

Species:		mouse

WB Figures

ESR2 Antibody WB human brain tissue 14007-1-AP

human brain tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MCF-7 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

ESR2 Antibody WB mouse brain tissue 14007-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP( ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ESR2 Antibody WB mouse testis tissue 14007-1-AP

mouse testis tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP( ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

PC-3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ESR2 Antibody WB SH-SY5Y cells 14007-1-AP

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human cervix using 14007-1-AP(ESR2 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human cervix using 14007-1-AP(ESR2 antibody) at dilution of 1:50 (under 40x lens)

ESR2 Antibody IHC human ovary tumor tissue 14007-1-AP

Immunohistochemical of paraffin-embedded human ovary tumor using 14007-1-AP(ESR2 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human ovary tumor using 14007-1-AP(ESR2 antibody) at dilution of 1:50 (under 40x lens)

ESR2 Antibody 15 Publications

Rabbit Polyclonal| Catalog number: 14007-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [6] of [15] Publications

WB Figures

IHC Figures

ESR2 Antibody 15 Publications

Rabbit Polyclonal| Catalog number: 14007-1-AP

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Related Primary Antibodies

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background