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  • KD/KO Validated

ESR2 Polyclonal antibody

ESR2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 14007-1-AP

Synonyms

ER BETA, Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2



Tested Applications

Positive WB detected inNIH/3T3 cells, human brain tissue, SH-SY5Y cells, mouse brain tissue, mouse testis tissue, PC-3 cells, MCF-7 cells
Positive IHC detected inhuman cervix tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14007-1-AP targets ESR2 in WB, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ESR2 fusion protein Ag5103
Full Name estrogen receptor 2 (ER beta)
Calculated molecular weight 59 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC024181
Gene symbol ESR2
Gene ID (NCBI) 2100
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa(PMID:14766008).

Protocols

Product Specific Protocols
WB protocol for ESR2 antibody 14007-1-APDownload protocol
IHC protocol for ESR2 antibody 14007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Hum Reprod

Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.

Authors - Yanling Gou
mouseWB

Heliyon

Establishment and evaluation of a novel mouse model of peri/postmenopausal depression.

Authors - Ling Zhang
WB

Mol Med Rep

Saikosaponin‑D improves fear memory deficits in ovariectomized rats via the action of estrogen receptor‑α in the hippocampus.

Authors - Lina Liu
mouse, humanWB

J Steroid Biochem Mol Biol

High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.

Authors - Rui Wang
ratWB

Biochem Cell Biol

Rutin attenuates cerebral ischemia/reperfusion injury in ovariectomized rats via estrogen receptor-mediated BDNF-TrkB and NGF-TrkA signaling.

Authors - Hong Liu
mouseWB

J Biomed Sci

Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.

Authors - Chunfang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kenneth (Verified Customer) (01-08-2020)

N/A

  • Applications: Western Blot,