ESR2 Polyclonal antibody

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  WB analysis using 14007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14007-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14007-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14007-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP (ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 14007-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14007-1-AP (ESR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 14007-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14007-1-AP (ESR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

ESR2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14007-1-AP

Publications(68)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ChIP, ELISA

Synonyms

ER BETA, Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, Estrogen Receptor β, NR3A2

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Tested Applications

• Positive WB detected in: MCF-7 cells, SKOV-3 cells, mouse testis tissue, mouse brain tissue, PC-3 cells, SH-SY5Y cells, NIH/3T3 cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 49 publications below
• IHC: See 29 publications below
• IF: See 9 publications below
• ChIP: See 1 publications below

Product Information

14007-1-AP targets ESR2 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ESR2 fusion protein Ag5103
• Full Name: estrogen receptor 2 (ER beta)
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC024181
• Gene Symbol: ESR2
• Gene ID (NCBI): 2100
• RRID: AB_2102386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92731
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoforms and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa(PMID:14766008).

Publications

Species	Application	Title
mouse	WB	J Biomed Sci; Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.; Authors - Chunfang Zhang
human	IF	ACS Sens; Estrogen Receptor β-Targeted Near-Infrared Inherently Fluorescent Probe: A Potent Tool for Estrogen Receptor β Research.; Authors - Qiuyu Meng
human	WB,IHC	Int J Cancer; ERβ promoted invadopodia formation-mediated non-small cell lung cancer metastasis via the ICAM1/p-Src/p-Cortactin signaling pathway; Authors - Yangwei Wang ; KD Validated
mouse	IHC	Int J Cancer; Estrogen and insulin-like growth factor synergistically promote the development of lung adenocarcinoma in mice.; Authors - Tang Hexiao H
human	WB	Cell Death Dis; ERβ promotes Aβ degradation via the modulation of autophagy.; Authors - Yong Wei
human	WB,IHC	Fertil Steril; Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.; Authors - Miao Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anna (Verified Customer) (10-04-2022)

No specific reaction - positive reaction should be visible only in nucleus but in our staining is present also in the cytoplasm. Moreover, we used the material from knockout mice without ESR2 and we observed response in this slices to this receptor. I have a problem with attaching a photo file. Please check the file below. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view

• Applications: Immunofluorescence
• Primary Antibody Dilution: 100, 200
• Cell Tissue Type: brain frozen sections - mouse

FH

Kenneth (Verified Customer) (01-08-2020)

N/A

• Applications: Western Blot,