|Positive WB detected in
|MCF-7 cells, SKOV-3 cells, mouse testis tissue, mouse brain tissue, PC-3 cells, SH-SY5Y cells, NIH/3T3 cells
|Positive IHC detected in
|mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:6000
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14007-1-AP targets ESR2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|ESR2 fusion protein Ag5103
|estrogen receptor 2 (ER beta)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoforms and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa(PMID:14766008).
J Biomed Sci
Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.
Estrogen Receptor β-Targeted Near-Infrared Inherently Fluorescent Probe: A Potent Tool for Estrogen Receptor β Research.
Int J Cancer
ERβ promoted invadopodia formation-mediated non-small cell lung cancer metastasis via the ICAM1/p-Src/p-Cortactin signaling pathway
Int J Cancer
Estrogen and insulin-like growth factor synergistically promote the development of lung adenocarcinoma in mice.
Cell Death Dis
ERβ promotes Aβ degradation via the modulation of autophagy.
Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Anna (Verified Customer) (10-04-2022)
No specific reaction - positive reaction should be visible only in nucleus but in our staining is present also in the cytoplasm. Moreover, we used the material from knockout mice without ESR2 and we observed response in this slices to this receptor. I have a problem with attaching a photo file. Please check the file below. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view
Kenneth (Verified Customer) (01-08-2020)