ESR2 Antibody 14007-1-AP

Tested Applications

Applications:

ELISA, IHC, WB
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Immunohistochemical of paraffin-embedded human cervix using 14007-1-AP(ESR2 antibody) at dilution of 1:50 (under 10x lens)

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP( ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Species specificity:

human, mouse, rat

Positive WB detected in:

NIH/3T3 cells, human brain tissue, mouse brain tissue, mouse testis tissue, NIH/3T3 cells, PC-3 cells, SH-SY5Y cells

Positive IHC detected in:

human cervix tissue, human cervix tissue, human ovary tumor tissue

Recommended dilution:

WB : 1:1000-1:4000

IHC : 1:20-1:200

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ESR2 fusion protein Ag5103

Full name:

estrogen receptor 2 (ER beta)

Calculated molecular weight:

59 kDa

Observed molecular weight:

55-60 kDa

GenBank accession number:

BC024181

Gene ID (NCBI):

2100

Gene symbol

ESR2

Synonyms

ER BETA, Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

Background

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa(PMID:14766008).

Showing 1- [6] of [9] Publications

Author:		Nan Zheng
Pubmed ID:		26767948
Journal:		Arch Biochem Biophys
Title:		ERβ up-regulation was involved in silibinin-induced growth inhibition of human breast cancer MCF-7 cells.
Application:		WB
KD validated
Species:		human

Author:		Lingmin Song
Pubmed ID:		27483178
Journal:		Bosn J Basic Med Sci
Title:		Differential expression of androgen, estrogen, and progesterone receptors in benign prostatic hyperplasia.
Application:		WB,IHC

Species:		human

Author:		Lingmin Song
Pubmed ID:		27294569
Journal:		Bosn J Basic Med Sci
Title:		Differential expression of androgen, estrogen, and progesterone receptors in benign prostatic hyperplasia.
Application:		WB,IHC

Species:		human

Author:		Jae Yoon Choi
Pubmed ID:		27873496
Journal:		Yonsei Med J
Title:		Ovarian Clear Cell Carcinoma Sub-Typing by ARID1A Expression.
Application:		IHC

Species:		human

Author:		He Yu-qi YQ
Pubmed ID:		23143558
Journal:		Exp Mol Med
Title:		Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells.
Application:		WB

Species:		human

Author:		Tang Hexiao H
Pubmed ID:		23649836
Journal:		Int J Cancer
Title:		Estrogen and insulin-like growth factor synergistically promote the development of lung adenocarcinoma in mice.
Application:		IHC

Species:		mouse

WB Figures

human brain tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP( ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

mouse testis tissue were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP( ESR2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

PC-3 cells were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14007-1-AP(ESR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human cervix using 14007-1-AP(ESR2 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human cervix using 14007-1-AP(ESR2 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human ovary tumor using 14007-1-AP(ESR2 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human ovary tumor using 14007-1-AP(ESR2 antibody) at dilution of 1:50 (under 40x lens)

ESR2 Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 14007-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [6] of [9] Publications

WB Figures

IHC Figures

ESR2 Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 14007-1-AP

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Tested Applications

Product Information

Immunogen Information

Background