Tested Applications
| Positive WB detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10684-1-AP targets ETV4 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0984 Product name: Recombinant human ETV4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-207 aa of BC007242 Sequence: MYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPFGPKGGYSY Predict reactive species |
| Full Name | ets variant 4 |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 61-65 kDa |
| GenBank Accession Number | BC007242 |
| Gene Symbol | ETV4 |
| Gene ID (NCBI) | 2118 |
| RRID | AB_2100984 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43268 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ETV4 antibody 10684-1-AP | Download protocol |
| WB protocol for ETV4 antibody 10684-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ETV4 Polyclonal antibody (Cat# 10684-1-AP) has 43 citations across journals including Cell, Nature Communications, Cancer Research, Science Advances, Oncogene, Cell Reports, Development, and others. Research fields span oncology (colorectal, lung, prostate, glioblastoma, sarcoma, melanoma), neurodegeneration, developmental biology (lung, kidney, spermatogenesis), ferroptosis, and immunology. Applications include WB, IHC, IF, and ChIP.
| Species | Application | Title |
|---|---|---|
Cell Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription. | ||
Mol Cancer Proteogenomic characterization of molecular and cellular targets for treatment-resistant subtypes in locally advanced cervical cancers | ||
Nat Commun Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5. | ||
Nat Commun The capicua-ataxin-1-like complex regulates Notch-driven marginal zone B cell development and sepsis progression | ||
J Clin Invest Targeting CIC::DUX4 sarcoma with Minnelide in a dual recombinase-initiated genetically engineered mouse model. |
