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ETV4 Polyclonal antibody

ETV4 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 10684-1-AP

Synonyms

E1A F, E1AF, ETS translocation variant 4, ets variant 4, ETV4, PEA3, PEAS3, Protein PEA3



Tested Applications

Positive WB detected inmouse heart tissue, BxPC-3 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10684-1-AP targets ETV4 in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ETV4 fusion protein Ag0984
Full Name ets variant 4
Calculated molecular weight 54 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC007242
Gene symbol ETV4
Gene ID (NCBI) 2118
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.

Protocols

Product Specific Protocols
WB protocol for ETV4 antibody 10684-1-APDownload protocol
IF protocol for ETV4 antibody 10684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

PPP6C negatively regulates oncogenic ERK signaling through dephosphorylation of MEK.

Authors - Eunice Cho
mouseIF

Cell Rep

RHOX10 drives mouse spermatogonial stem cell establishment through a transcription factor signaling cascade.

Authors - Kun Tan
humanWB

Cell Biol Int

ETV4 transcriptionally activates HES1 and promotes Stat3 phosphorylation to promote malignant behaviors of colon adenocarcinoma.

Authors - Dan Yao
humanWB

Cancer Cell Int

Capicua suppresses colorectal cancer progression via repression of ETV4 expression.

Authors - Jeon-Soo Lee
chickenWB,IHC

Cells

The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine.

Authors - Aoi Komatsu
humanIF

Development

Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney.

Authors - Rajasree Menon