EVC2 Polyclonal antibody, PBS Only

• 

  WB analysis using 55367-1-AP (same clone as 55367-1-PBS)

  Mouse lung tissue were subjected to SDS PAGE followed by western blot with 55367-1-AP (EVC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55367-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 55367-1-AP (same clone as 55367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55367-1-AP (EVC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55367-1-PBS in a different storage buffer formulation.

EVC2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 55367-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

55367-1-PBS targets EVC2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Ellis van Creveld syndrome 2
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_147127
• Gene Symbol: EVC2
• Gene ID (NCBI): 132884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86UK5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

EVC2 (also known as LIMBIN) is close proximity in divergent orientation to EVC and mutations in both human genes lead to the chondrodysplasia Ellis-van Creveld (EvC) syndrome. Typical symptoms of EvC include shorter limbs and ribs, postaxial polydactyly, dysplastic nails, and cardiovascular defects (PMID: 28081373). Additionally, EvC syndrome has been characterized as a ciliopathy because the EVC and EVC2 proteins are intracellularly localized at the bottom of the primary cilium, where they form a heterotrimeric protein complex with SMO and transduce Hedgehog(Hh) signaling (PMID: 30410447). EVC2 is expressed in the heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. EVC2 has 3 isoforms with the molecular mass of 140-148 kDa.