Validation Data Gallery
|Positive WB detected in||mouse brain tissue, human adrenal gland tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human testis tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17593-1-AP targets EXOC5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||EXOC5 fusion protein Ag11524|
|Full Name||exocyst complex component 5|
|Calculated molecular weight||708 aa, 82 kDa|
|Observed molecular weight||77 kDa|
|GenBank accession number||BC041126|
|Gene ID (NCBI)||10640|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
EXOC5 (SEC10), a component of the exocyst complex, is a 77-kDa protein with a broad tissue distribution (PubMed: 9119050). The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. The complex is also essential for the biogenesis of epithelial cell surface polarity.
|Product Specific Protocols|
|WB protocol for EXOC5 antibody 17593-1-AP||Download protocol|
|IHC protocol for EXOC5 antibody 17593-1-AP||Download protocol|
|IF protocol for EXOC5 antibody 17593-1-AP||Download protocol|
|IP protocol for EXOC5 antibody 17593-1-AP||Download protocol|
|Click here to view our Standard Protocols|
RalB and the exocyst mediate the cellular starvation response by direct activation of autophagosome assembly.
Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis
Insights into the ubiquitin-proteasome system of human embryonic stem cells.
Mol Cancer Res
EXOC4 Promotes Diffuse-Type Gastric Cancer Metastasis via Activating FAK Signal.
The Exocyst Is Required for CD36 Fatty Acid Translocase Trafficking and Free Fatty Acid Uptake in Skeletal Muscle Cells
J Biol Chem
Disrupted glucose homeostasis and skeletal muscle-specific glucose uptake in an exocyst knockout mouse model.