Tested Applications
| Positive WB detected in | human adipose tissue, MC38 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse adipose tissue, mouse brown adipose tissue, mouse heart tissue, rat adipose tissue |
| Positive IHC detected in | human colon tissue, human cervix tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse adipose, HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15872-1-AP targets FABP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8631 Product name: Recombinant human FABP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-132 aa of BC003672 Sequence: VGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA Predict reactive species |
| Full Name | fatty acid binding protein 4, adipocyte |
| Calculated Molecular Weight | 132 aa, 15 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC003672 |
| Gene Symbol | FABP4 |
| Gene ID (NCBI) | 2167 |
| RRID | AB_2102440 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15090 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FABP4 antibody 15872-1-AP | Download protocol |
| IHC protocol for FABP4 antibody 15872-1-AP | Download protocol |
| WB protocol for FABP4 antibody 15872-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FABP4 antibody (Cat# 15872-1-AP) has 17 citations published in journals including Cell, European Heart Journal, Autophagy, Developmental Cell, Cell Reports, PLoS Biology, Cell Molecular Life Sciences, and others. Associated research fields span cardiovascular disease, adipocyte biology and differentiation, cellular aging, neuroinflammation, cancer (breast, bladder), autophagy, wound healing, lymphatic endothelial cell biology, sepsis, peripheral neuropathy, and reproductive physiology.
| Species | Application | Title |
|---|---|---|
Eur Heart J Adipocyte fatty acid binding protein in atherosclerotic plaques is associated with local vulnerability and is predictive for the occurrence of adverse cardiovascular events. | ||
Autophagy Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis. | ||
J Neuroinflammation Fatty acid-binding protein 4 drives microglia-mediated neuroinflammation through promoting S100A9 expression and lipid droplet accumulation after intracerebral hemorrhage. | ||
Dev Cell The p75 neurotrophin receptor controls the skeletal stem cell niche through sensory innervation | ||
Cell Rep Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration |
