Tested Applications
| Positive WB detected in | HeLa cells, SGC-7901 cells, hTERT-RPE1 cells |
| Positive IHC detected in | human skin tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15939-1-AP targets Piezo1 (extracellular domain) in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, canine, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7791 Product name: Recombinant human FAM38A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 67-373 aa of BC008073 Sequence: SVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPQPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKHHGAVRVHRAGHRQVRARILQRDLALHYVRGAAVRGPHPQALPGHLPGAGDSGAGAGGGVVCQAHLPLPLTGDHDQVDS Predict reactive species |
| Full Name | family with sequence similarity 38, member A |
| Calculated Molecular Weight | 286 kDa |
| Observed Molecular Weight | 233-286 kDa |
| GenBank Accession Number | BC008073 |
| Gene Symbol | Piezo1/FAM38A |
| Gene ID (NCBI) | 9780 |
| RRID | AB_2231460 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92508 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Piezo1 (extracellular domain) antibody 15939-1-AP | Download protocol |
| IHC protocol for Piezo1 (extracellular domain) antibody 15939-1-AP | Download protocol |
| WB protocol for Piezo1 (extracellular domain) antibody 15939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Piezo1 antibody (Cat# 15939-1-AP) has accumulated 303 citations across prestigious journals including Nature, Cell, Immunity, PNAS, Nat Commun, J Clin Invest, Blood, Neuron, Sci Transl Med, and Adv Mater. Associated research fields span mechanobiology, vascular biology, bone biology, oncology, immunology, neuroscience, hematology, pulmonary disease, fibrosis, ferroptosis, and wound healing, reflecting its broad utility in studying mechanosensitive ion channel biology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Piezo1 activation suppresses bone marrow adipogenesis to prevent osteoporosis by inhibiting a mechanoinflammatory autocrine loop.
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Nature Mechanical stretch triggers rapid epithelial cell division through Piezo1.
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Nature Skin cells undergo asynthetic fission to expand body surfaces in zebrafish.
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Nature Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia.
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Reviews
What customers say
Across 10 reviews, users report generally positive results for WB, IHC, and IF. Western blot performance is the most discussed: several note the large protein size makes sharp, clean bands challenging, and one user found the signal weak with HRP/chemiluminescence and undetectable with NIR-conjugated secondaries. One reviewer did not observe the expected band. IHC in kidney tissue showed staining matching the expected pattern. For IF, one user observed non-specific staining in knockout cells, while another described results as clean and specific. Overall, the antibody is considered reliable for detecting the target at the correct molecular weight, though optimization may be needed for WB due to the large protein size.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Yao (Verified Customer) (09-24-2025) | I used this antibody for the kidney slides of wild-type mice, it showed positive staining that matches the expected pattern
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Hyun (Verified Customer) (07-23-2025) | This antibody works well for western blot.
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Jason (Verified Customer) (02-11-2025) | The antibody works well. However, due to the large protein size, obtaining sharp and clean Western blot bands is challenging for me. In fluorescence staining, the knockout cells were stained similarly to the control cells, suggesting non-specific staining in IF.
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Hyojin (Verified Customer) (01-19-2024) | I like the quality of antibody. Thank you for generating.
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Sammy (Verified Customer) (12-04-2023) | Ab detects a band at the correct size but the signal is weak. This works only when detected via HRP/Chemi. NIR-conjugated antibodies do not detect this band.
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Xiaonan (Verified Customer) (02-01-2023) | We did not see the band for Piezol.
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LUNFENG (Verified Customer) (01-27-2020) | GOOD
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Jie (Verified Customer) (01-23-2020) | Works with one band. Due to the large protein size, band does not always show sharp image.
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Canzhao (Verified Customer) (01-17-2020) | It worked in western blot and showed nice band.
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Zeinab (Verified Customer) (09-27-2019) | Worked great, Clean and Specific
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