Piezo1 (extracellular domain) Polyclonal antibody

• Featured Product
• KD/KO Validated

Piezo1 (extracellular domain) Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

96

Reviews (5)

Cat no : 15939-1-AP

Synonyms

FAM38A, KIAA0233, Mib, PIEZO1, Piezo1 (extracellular domain)

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Validation Data Gallery

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  WB analysis of HeLa using 15939-1-AP

  WB result of Piezo1 antibody (15939-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-Piezo1 transfected HeLa cells.

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  WB analysis using 15939-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15939-1-AP (Piezo1 (extracellular domain) antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15939-1-AP (Piezo1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 15939-1-AP (Piezo1 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15939-1-AP (Piezo1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15939-1-AP (Piezo1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15939-1-AP (Piezo1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 15939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15939-1-AP (Piezo1 antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HUVEC using 15939-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 15939-1-AP (Piezo1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of RAW 264.7 using 15939-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human Piezo1 (extracellular domain) (15939-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, SGC-7901 cells, hTERT-RPE1 cells
• Positive IHC detected in: human skin tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HUVEC cells
• Positive FC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 28 publications below
• WB: See 70 publications below
• IHC: See 23 publications below
• IF: See 49 publications below
• FC: See 2 publications below
• CoIP: See 3 publications below

Product Information

15939-1-AP targets Piezo1 (extracellular domain) in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, canine, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Piezo1 (extracellular domain) fusion protein Ag7791
• Full Name: family with sequence similarity 38, member A
• Calculated molecular weight: 286 kDa
• Observed molecular weight: 233-286 kDa
• GenBank accession number: BC008073
• Gene symbol: FAM38A
• Gene ID (NCBI): 9780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)

Publications

Species	Application	Title
zebrafish	IF	Nature; Skin cells undergo asynthetic fission to expand body surfaces in zebrafish.; Authors - Keat Ying Chan ; KO Validated
mouse,zebrafish	WB	Nature; Crowding induces live cell extrusion to maintain homeostatic cell numbers in epithelia.; Authors - Eisenhoffer George T GT ; KD Validated
human	WB	Nature; Piezo1 integration of vascular architecture with physiological force.; Authors - Jing Li ; KD Validated
canine, zebrafish	WB,IF	Nature; Mechanical stretch triggers rapid epithelial cell division through Piezo1.; Authors - S A Gudipaty ; KO Validated
mouse	IHC	Cell; RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis.; Authors - Erika Sugisawa
human	WB	Blood; Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis.; Authors - ShengPeng Wang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaonan (Verified Customer) (02-01-2023)

We did not see the band for Piezol.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse kidney

Piezo1 (extracellular domain) Antibody Western Blot validation (1:1000 dilution) in mouse kidney (Cat no:15939-1-AP)

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,
• Cell Tissue Type: HEART

FH

Jie (Verified Customer) (01-23-2020)

Works with one band. Due to the large protein size, band does not always show sharp image.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela cells

FH

Canzhao (Verified Customer) (01-17-2020)

It worked in western blot and showed nice band.

• Applications: Western Blot,
• Cell Tissue Type: Mouse heart tissue

FH

Zeinab (Verified Customer) (09-27-2019)

Worked great, Clean and Specific

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Human Cardiac Microvascular Endothelial cells

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