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  • KD/KO Validated

Piezo1 (extracellular domain) Polyclonal antibody

Piezo1 (extracellular domain) Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF, FC, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 15939-1-AP

Synonyms

FAM38A, KIAA0233, Mib, PIEZO1



Tested Applications

Positive WB detected inHepG2 cells, human brain tissue, HUVEC cells, mouse lung tissue, HeLa cells, COLO 320 cells
Positive IHC detected inhuman skin tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15939-1-AP targets Piezo1 (extracellular domain) in WB, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity canine, human, mouse, pig, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Piezo1 (extracellular domain) fusion protein Ag7791
Full Name family with sequence similarity 38, member A
Calculated molecular weight 286 kDa
Observed molecular weight 233-286 kDa
GenBank accession numberBC008073
Gene symbol FAM38A
Gene ID (NCBI) 9780
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells which influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)

Protocols

Product Specific Protocols
WB protocol for Piezo1 (extracellular domain) antibody 15939-1-APDownload protocol
IHC protocol for Piezo1 (extracellular domain) antibody 15939-1-APDownload protocol
IF protocol for Piezo1 (extracellular domain) antibody 15939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Adv Mater

Mechanically Defined Microenvironment Promotes Stabilization of Microvasculature, Which Correlates with the Enrichment of a Novel Piezo-1+ Population of Circulating CD11b+ /CD115+ Monocytes.

Authors - Aurelien Forget
mouseIF

Nat Commun

Oscillatory cortical forces promote three dimensional cell intercalations that shape the murine mandibular arch.

Authors - Hirotaka Tao
mouseWB,IF

Am J Physiol Cell Physiol

Piezo1 mediates angiogenesis through activation of MT1-MMP signaling.

Authors - Hojin Kang
  • KO Validated
mouseIF

Am J Respir Cell Mol Biol

Stretch-Activated Piezo1 Channel in Endothelial Cells Relaxes Mouse Intrapulmonary Arteries.

Authors - Audrey Lhomme
  • KO Validated
humanIHC

Cancer Manag Res

Upregulation of Piezo1 Is a Novel Prognostic Indicator in Glioma Patients.

Authors - Shanqiang Qu
humanWB,FC

Int J Mol Sci

Mechanical Stress Induces Ca2+-Dependent Signal Transduction in Erythroblasts and Modulates Erythropoiesis.

Authors - Francesca Aglialoro

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jie (Verified Customer) (01-23-2020)

Works with one band. Due to the large protein size, band does not always show sharp image.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hela cells
FH

Canzhao (Verified Customer) (01-17-2020)

It worked in western blot and showed nice band.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse heart tissue
FH

Zeinab (Verified Customer) (09-27-2019)

Worked great, Clean and Specific

  • Applications: Western Blot,Immunofluorescence,
  • Cell Tissue Type: Human Cardiac Microvascular Endothelial cells