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  • KD/KO Validated

Piezo1 (extracellular domain) Polyclonal antibody

Piezo1 (extracellular domain) Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (4)





Cat no : 15939-1-AP


FAM38A, KIAA0233, Mib, PIEZO1, Piezo1 (extracellular domain)

Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, HUVEC cells, HepG2 cells
Positive IHC detected inhuman skin tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15939-1-AP targets Piezo1 (extracellular domain) in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, canine, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Piezo1 (extracellular domain) fusion protein Ag7791
Full Name family with sequence similarity 38, member A
Calculated molecular weight 286 kDa
Observed molecular weight 233-286 kDa
GenBank accession numberBC008073
Gene symbol FAM38A
Gene ID (NCBI) 9780
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells which influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)


Product Specific Protocols
WB protocol for Piezo1 (extracellular domain) antibody 15939-1-APDownload protocol
IHC protocol for Piezo1 (extracellular domain) antibody 15939-1-APDownload protocol
IF protocol for Piezo1 (extracellular domain) antibody 15939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Mater

Mechanically Defined Microenvironment Promotes Stabilization of Microvasculature, Which Correlates with the Enrichment of a Novel Piezo-1+ Population of Circulating CD11b+ /CD115+ Monocytes.

Authors - Aurelien Forget

Nat Commun

Oscillatory cortical forces promote three dimensional cell intercalations that shape the murine mandibular arch.

Authors - Hirotaka Tao

Nat Commun

A critical role of the mechanosensor PIEZO1 in glucose-induced insulin secretion in pancreatic β-cells.

Authors - Yingying Ye

Respir Res

Mechanosensitive cation channel Piezo1 contributes to ventilator-induced lung injury by activating RhoA/ROCK1 in rats.

Authors - Yang Zhang

JCI Insight

Mechanosensitive Piezo1 channels mediate renal fibrosis.

Authors - Xiaoduo Zhao

Am J Physiol Cell Physiol

Piezo1 mediates angiogenesis through activation of MT1-MMP signaling.

Authors - Hojin Kang
  • KO Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


LUNFENG (Verified Customer) (01-27-2020)


  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART

Jie (Verified Customer) (01-23-2020)

Works with one band. Due to the large protein size, band does not always show sharp image.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hela cells

Canzhao (Verified Customer) (01-17-2020)

It worked in western blot and showed nice band.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse heart tissue

Zeinab (Verified Customer) (09-27-2019)

Worked great, Clean and Specific

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Human Cardiac Microvascular Endothelial cells