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Piezo1 (extracellular domain) Polyclonal antibody
Piezo1 (extracellular domain) Polyclonal Antibody for IF, IHC, WB,ELISA
Cat no : 15939-1-AP
|Positive WB detected in||HepG2 cells, human brain tissue, HUVEC cells, mouse lung tissue, HeLa cells, COLO 320 cells|
|Positive IHC detected in||human skin tissue, human skin cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HUVEC cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15939-1-AP targets Piezo1 (extracellular domain) in WB, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||canine, human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Piezo1 (extracellular domain) fusion protein Ag7791|
|Full Name||family with sequence similarity 38, member A|
|Calculated molecular weight||286 kDa|
|Observed molecular weight||233-286 kDa|
|GenBank accession number||BC008073|
|Gene ID (NCBI)||9780|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells which influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)
|Product Specific Protocols|
|WB protocol for Piezo1 (extracellular domain) antibody 15939-1-AP||Download protocol|
|IHC protocol for Piezo1 (extracellular domain) antibody 15939-1-AP||Download protocol|
|IF protocol for Piezo1 (extracellular domain) antibody 15939-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mechanically Defined Microenvironment Promotes Stabilization of Microvasculature, Which Correlates with the Enrichment of a Novel Piezo-1+ Population of Circulating CD11b+ /CD115+ Monocytes.
Oscillatory cortical forces promote three dimensional cell intercalations that shape the murine mandibular arch.
Am J Physiol Cell Physiol
Piezo1 mediates angiogenesis through activation of MT1-MMP signaling.
Am J Respir Cell Mol Biol
Stretch-Activated Piezo1 Channel in Endothelial Cells Relaxes Mouse Intrapulmonary Arteries.
Cancer Manag Res
Upregulation of Piezo1 Is a Novel Prognostic Indicator in Glioma Patients.
Int J Mol Sci
Mechanical Stress Induces Ca2+-Dependent Signal Transduction in Erythroblasts and Modulates Erythropoiesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
LUNFENG (Verified Customer) (01-27-2020)
Jie (Verified Customer) (01-23-2020)
Works with one band. Due to the large protein size, band does not always show sharp image.
Canzhao (Verified Customer) (01-17-2020)
It worked in western blot and showed nice band.
Zeinab (Verified Customer) (09-27-2019)
Worked great, Clean and Specific